In vitro and in vivo synergistic antifungal effects of microbially synthesized silver and magnetic iron oxide nanoparticles against Candida albicans

被引：0
作者：
Bahmaee, Fatemeh [1 ]
Khodavandi, Pouria [2 ]
Khodavandi, Alireza [1 ]
Alizadeh, Fahimeh [1 ]
机构：
[1] Islamic Azad Univ, Dept Chem Engn Gac C, Gachsaran, Iran
[2] Shiraz Univ, Dept Anim Sci, Shiraz, Iran
来源：
INORGANIC CHEMISTRY COMMUNICATIONS | 2025年 / 180卷
关键词：
Candida albicans; Galleria mellonella; Magnetic iron oxide nanoparticles; Silver nanoparticles; RESISTANT CANDIDA; FLUCONAZOLE; ANTIBACTERIAL;
D O I：
10.1016/j.inoche.2025.115093
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
Nanobiotechnology-based antifungal strategies offer promising biomedical applications. This study explores the synergistic antifungal effects of microbially synthesized silver (nano-Ag) and magnetic iron oxide (nano-Fe3O4) nanoparticles against Candida albicans, including fluconazole-resistant isolates. Nano-Ag-2 and nano-Fe3O4-10 were biosynthesized using Bacillus licheniformis and Fusarium oxysporum, respectively. The combination of nanoAg and nano-Fe3O4 effectively inhibited biofilm formation, disrupted fungal membrane integrity by targeting ergosterol biosynthesis, and downregulated ERG1, ERG3, and ERG11 expression. The Galleria mellonella model confirmed their therapeutic potential, demonstrating reduced fungal burden and enhanced immune responses via haemocyte activation. In addition, the combination of these nanoparticles with fluconazole restored antifungal activity against resistant isolates. These findings highlight the potential of nano-Ag and nano-Fe3O4 as novel antifungal agents that act through multiple mechanisms, including immune modulation and direct antifungal activity. Targeting ergosterol biosynthesis genes with nano-Ag and nano-Fe3O4, alone or in combination, may be an effective novel therapeutic approach against C. albicans infections, particularly fluconazole-resistant isolates.
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页数：17
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