Protective effect of methylene blue on neuronal damage induced by okadaic acid in differentiated SK-N-SH neuroblastoma cells

被引：0

作者：

Si Y. ^{[1
]}

Yuan Y. ^{[2
]}

Qiu L. ^{[2
]}

Lü H. ^{[3
]}

Zhou H. ^{[2
]}

Xu B. ^{[1
]}

Li G. ^{[1
]}

Li J. ^{[1
]}

Wang Z. ^{[1
]}

机构：

[1] Center of Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai

[2] School of Pharmacy, Shihezi University, Shihezi

[3] Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining

来源：

Shipin Kexue/Food Science | 2016年 / 37卷 / 17期

关键词：

All-trans retinoic acid; Differentiated SK-N-SH neuroblastoma cells; Methylene blue; Okadaic acid; Tau phosphorylation;

D O I：

10.7506/spkx1002-6630-201617033

中图分类号：

学科分类号：

摘要：

Objective: To investigate the protective effect and underlying mechanism of methylene blue (MB) on neuronal damage induced by okadaic acid (OA). Methods: The mature neurons differentiated from SK-N-SH neuroblastoma cells induced by all-trans retinoic acid (ATRA) were exposed to OA to induce synaptic atrophy and cell injury. The SRB assay was used to measure the effect of MB on the proliferation of differentiated SK-N-SH cells. Giemsa staining was used to observe cell morphology. The expression of synaptophysin and the phosphorylation of tau protein were detected by Western blotting. Results: ATRA inhibited the proliferation of SK-N-SH cells in a dose-dependent manner in vitro. The 10 μmol/L ATRA exposure for 7 days could induce SK-N-SH neuroblastoma cells differentiation into mature neurons with obviously extended synapse. Treatment with 40 nmol/L OA resulted in synaptic atrophy in differentiated SK-N-SH neuroblastoma cells. MB inhibited the proliferation of differentiated SK-N-SH cells in a dose-dependent manner in vitro. Moreover, MB treatment significantly increased the ratio of axon length to cell body length and the expression of synaptophysin (P < 0.01), while it decreased the phosphorylation of tau protein at Ser262 site (P < 0.01). Conclusion: MB can alleviate synaptic atrophy induced by OA, which may be due to the down-regulation of tau phosphorylation at Ser262 site. © 2016, China Food Publishing Company. All right reserved.

引用

页码：195 / 200

页数：5

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