Effects of microsecond pulsed magnetic field with different magnetic induction intensities on ovarian cancer cell line SKOV3

被引：0

作者：

Mi Y. ^{[1
]}

Rui S. ^{[1
,3
]}

Chu Y. ^{[1
,4
]}

Zhang Y. ^{[1
]}

Tang J. ^{[2
]}

Long Y. ^{[2
]}

机构：

[1] State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing

[2] First Affiliated Hospital, Chongqing Medical Science University, Chongqing

[3] Maintenance Company, State Grid Tianjin Electric Power Company, Tianjin

[4] Maintenance Company, State Grid Anhui Electric Power Company, Hefei

来源：

Gaodianya Jishu/High Voltage Engineering | 2016年 / 42卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Bioelectromagnetic effects; Flow cytometry method; Magnetic induction intensity; Microsecond pulsed magnetic field; MTT; Ovarian cancer cell SKOV3;

D O I：

10.13336/j.1003-6520.hve.20160812006

中图分类号：

学科分类号：

摘要：

The damage of lightning pulsed electromagnetic fields becomes more and more serious, but few people study the impact of this microsecond pulsed electromagnetic field on people. In order to study the bioelectromagnetic mechanism of microsecond pulsed magnetic field and its effects on human health, human ovarian cancer cell line SKOV3 was used as subjects. A magnetic field pulse generator was adopted to generate a pulse magnetic field of different magnetic induction intensity (magnetic induction intensity of 1~200 mT, rise time of 800 ns, the pulse width of 8.4μs, a frequency of 8 Hz) to treat cells 3 days, 12 hours interval per treatment, 1 hour each time. The MTT assay and FCM were used to detect the proliferation inhibition and apoptosis rate 24 hours after the end of treatment. The results show that the proliferation inhibition rate did not show the trend of change with magnetic induction intensity of pulse magnetic field, and the values of proliferation inhibition rate were all less than 5%. Microsecond pulse magnetic field had no significant effects on cell proliferation inhibition rate of SKOV3 (P>0.05); tumor cells apoptosis rate did not show the trend of change with magnetic induction intensity of pulse magnetic field, and the values were all lower than 12%. Microsecond pulse magnetic field had no significant effect on apoptosis of SKOV3 cells (P>0.05). The results reveal that different intensities of magnetic induction (1~200 mT) of microsecond pulsed magnetic field have no significant effects on ovarian cancer SKOV3. © 2016, High Voltage Engineering Editorial Department of CEPRI. All right reserved.

引用

页码：2402 / 2408

页数：6

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