Induction mechanism of genistein on Vtg mRNA in male zebrafish (Danio rerio)

被引：0
作者：
Wang, Dexin [1 ]
Chen, Jie [1 ]
Wang, Wei [1 ]
Ru, Shaoguo [1 ]
机构：
[1] Marine Life Science College, Ocean University of China, Qingdao
来源：
| 2015年 / 28卷 / 01期
关键词：
ERs (estrogen receptors); Genistein; Vtg (vitellogenin); Zebrafish;
D O I：
10.13198/j.issn.1001-6929.2015.01.06
中图分类号：
学科分类号：
摘要：
Fish species are being threaten by the high concentration of genistein in water bodies. It is urgent to explore the estrogenic effects and the mechanisms of genistein for aquaculture development and fishery resources protection. Mature individuals of male zebrafish were exposed to a range of concentrations of genistein (1, 10, 100, 1000 μg/L) for 28 days. The study was performed using real-time PCR as a readout to quantify the change in vitellogenin (Vtg-1) mRNA levels. ERα and ERβ1 mRNA levels were then quantified by real-time PCR to explore the action mechanism of genistein. Meanwhile, radioimmunoassay was used to detect the change of E2 (17β-estradiol) and testosterone levels in gonad of male individuals. The results showed that exposures to 10-1000 μg/L genistein led to dose-dependent increase in Vtg-1 mRNA. The transcriptions of ERβ1 mRNA were significantly stimulated, while the transcriptions of ERα mRNA were significantly inhibited when exposed to 1-1000 μg/L genistein. In addition, genistein treatments enhanced the levels of E2 and reduced the testosterone levels. The results suggest that genistein may enhance the synthesis of Vtg through increasing endogenous E2 and ERβ1 levels and thus improve the mediation of ERβ1 for endogenous E2. ©, 2015, Editorial department of Molecular Catalysis. All right reserved.
引用
收藏
页码：46 / 51
页数：5
相关论文
共 31 条
[1]  
Han F., Zhang Y., Yang H., The content determination and the analyse of isoflavones component in soy products, Soybean Bulletin, 5, pp. 20-23, (2006)
[2]  
Sun J., Sun B., Han F., Et al., A rapad HPLC method for determination of 12 isoflavone components in soybean seeds, Scientia Agriculturea Sinica, 42, 7, pp. 2491-2498, (2009)
[3]  
Kiparissis Y., Hughes R., Metcalfe C., Et al., Identification of the isoflavonoid genistein in bleached kraft mill effluent, Environmental Science and Technology, 35, 12, pp. 2423-2427, (2001)
[4]  
Kawanishi M., Takamura-Enya T., Ermawati R., Et al., Detection of genistein as an estrogenic contaminant of river water in Osaka, Environmental Science & Technology, 38, 23, pp. 6424-6429, (2004)
[5]  
Ishibashi H., Kobayashi M., Koshiishi T., Et al., Induction of plasma vitellogenin synthesis by the commercial fish diets in male goldfish(Carassius auratus) and dietary phytoestrogens, Journal of Health Science, 48, 5, pp. 427-434, (2002)
[6]  
Yuan J., Wang J., Liu X., Research advance on the biological activities of soybean isoflavones:(1) metabolism and estrogenic behaviors of soybean isoflavones, Journal of Chinese Institute of Food Science and Technology, 3, 3, pp. 81-85, (2003)
[7]  
Bennetau-Pelissero C., Breton B.B., Bennetau B., Et al., Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss, General Comparative Endocrinology, 121, pp. 173-187, (2001)
[8]  
Scholz S., Kordes C., Hamann J., Et al., Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes):comparison of gene expression and protein levels, Marine Environmental Research, 57, pp. 235-244, (2004)
[9]  
Ng Y., Hanson S., Malison J.A., Et al., Genistein and other isoflavones found in soybeans inhibit estrogen metabolism in salmomid fish, Aquaculture, 254, pp. 658-665, (2006)
[10]  
Jourdehi A.Y., Sudagar M., Bahmani M., Et al., Comparative study of dietary soy phytoestrogens genistein and equol effects on growth parameters and ovarian development in farmed female beluga sturgeon, Huso huso, Fish Physiology Biochemistry, 40, pp. 117-128, (2014)
1234