Synthesis and study on properties of an magnetic resonance/fluorescence bifunctional imaging molecular probe using low degree of polymerization and narrow molecular weight distribution chitosan as carrier

被引：0

作者：

Liu, Chun-Ling ^{[1
]}

Jia, Chun-Man ^{[1
]}

Zhang, Qi ^{[1
]}

Qian, Ya-Ao ^{[1
]}

Zhang, De-La ^{[1
]}

Yang, Qiu-Yun ^{[1
]}

Yang, Yi-Xuan ^{[1
]}

机构：

[1] Hainan Provincial Key Lab of Fine Chemistry, Key Study Center of the National Ministry of Education for Tropical Resources Utilization, Hainan University, Haikou,Hainan,570228, China

来源：

Jingxi Huagong/Fine Chemicals | 2016年 / 33卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Polymerization - Body fluids - Molecular weight distribution - Molecular imaging - Chitosan - Probes - Magnetism - Fluorescence;

D O I：

10.13550/j.jxhg.2016.02.022

中图分类号：

学科分类号：

摘要：

Low degree of polymerization (DP) and narrow molecular weight distribution chitosan (LNCS6) (DP=6, Mw/Mn=1.08) was prepared with the method of microwave-assisted oxidative degradation. It was used as a carrier molecular to link 7-hydroxyl-8-formylcoumarin (CA) as the fluorescence emission group, diethylenetriaminepentaacetic acid (DTPA) as complex of gadolinium ions to get the final product Gd [DTPA/CA-LNCS6] as an potential magnetic resonance (MR)/fluorescence bifunctional imaging molecular probe. The magnetic resonance imaging (MRI) efficiency and fluorescence properties were studied, and the binding constants and thermodynamic parameters between Gd [DTPA/CA-LNCS6] and bovine serum albumin (BSA) were measured by isothermal titration calorimetry (ITC). The results showed that Gd [DTPA/CA-LNCS6] exhibited higher MRI relaxation [r1=13.71 L/(mmol·s)], which was well above the clinically used magnevist [r1=3.63 L/(mmol·s)]. Meanwhile, the product had significant fluorescence characteristics at 438 nm, and the binding constant between Gd [DTPA/CA-LNCS6] and BSA was over 104 which was suitable for blood transportation. This work has good reference for the synthesis of probes like such type of fine chemical intermediates. © 2016, Editorial Office of FINE CHEMICALS. All right reserved.

引用

页码：235 / 240