Current blockade mechanism for DNA translocation through solid-state nanopore with different membrane thickness

被引：0

作者：

Wu G. ^{[1
]}

Zhang Y. ^{[2
]}

Si W. ^{[2
]}

Gu Y. ^{[1
]}

Chen Y. ^{[2
]}

机构：

[1] School of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing

[2] Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing

来源：

Journal of Southeast University (English Edition) | 2016年 / 32卷 / 03期

关键词：

DNA sequencing; Membrane thickness; Nanopore; Relative current blockade;

D O I：

10.3969/j.issn.1003-7985.2016.03.007

中图分类号：

学科分类号：

摘要：

The current blockade mechanism for λ-DNA translocation under electrical field is investigated through solid-state nanopores with different pore thicknesses. The conductance of a nanopore system mainly consists of the contribution of the pore and access region, and the latter becomes dominant when the nanopore thickness gradually decreases to atomic layer thickness. Based on the existing model of nanopore resistance, a simplified model which describes the relative current blockade during the λ-DNA translocation through the nanopores is deduced to quantitatively present the relationship between nanopore thickness and relative current blockade. Results show that the relative current blockade is effectively increased by reducing the nanopore diameter but it decreases with the decreasing nanopore thickness. A two-stage schematic is proposed to increase the relative current blockade by setting a much smaller resistance region. Experimental results show a 21.9% increase in the relative current blockade with the proposed schematic. © 2016, Editorial Department of Journal of Southeast University. All right reserved.

引用

页码：301 / 306

页数：5

共 33 条

[31]

Goepfrich K., Kulkarni C.V., Pambos O.J., Et al., Lipid nanobilayers to host biological nanopores for DNA translocations, Langmuir, 29, 1, pp. 355-364, (2013)

[32]

Kasianowicz J.J., Brandin E., Branton D., Et al., Characterization of individual polynucleotide molecules using a membrane channel, Proceedings of the National Academy of Sciences, 93, 24, pp. 13770-13773, (1996)

[33]

Clarke J., Wu H.C., Jayasinghe L., Et al., Continuous base identification for single-molecule nanopore DNA sequencing, Nature Nanotechnology, 4, 4, pp. 265-270, (2009)

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