Complete Mitochondrial Genome and Its Phylogenetic Analysis of Oides decempunctatus (Coleoptera: Chrysomelidae)

Oides decempunctatus is an insect that parasitizes plants in the grape family and causes significant damage to grape buds and leaves. This study successfully determined the mitochondrial genome sequence of O. decempunctatus and analyzed its gene structure and the phylogenetic relationships within the family Chrysomelidae. Using the Illumina HiSeq platform, the complete mitochondrial genome of O. decempunctatus was sequenced to examine its genomic structural characteristics and nucleotide composition. Based on the sequences of 13 protein-coding genes and 2 rRNA genes, along with complete mitochondrial genome sequences of 24 Chrysomelidae species from GenBank, we selected two species from the subfamily Chrysomelinae, Chrysomela vigintipunctata and Chrysomela aeneicollis, as outgroups. Phylogenetic trees were constructed using maximum likelihood and Bayesian inference methods. The mitochondrial genome of O. decempunctatus is 16,062 base pairs long, circular, and double-stranded, containing 22 tRNA genes, 2 rRNA genes, 13 PCGs, and a control region, with an arrangement monophyletic group. Notably, within the subfamily Chrysomelinae, the species Gastrolina depressa and Gastrolina thoracica clustered with the subfamily Galerucinae in both analyses, forming branches with high node support values. Furthermore, O. decempunctatus and G. depressa were found to be sister groups with high support in both maximum likelihood and Bayesian analyses. This study provides important foundational information for further research on the mitochondrial genomes of Chrysomelidae.