Separation of lysozyme from egg white by electrodialysis with ultrafiltration membrane(EDUF) process

被引：0

作者：

Sun L. ^{[1
]}

Lu H. ^{[1
]}

Wang J. ^{[1
]}

机构：

[1] Tianjin Key Laboratory of Environmental Technology for Complex Trans-Media Pollution, College of Environmental Science and Engineering, Nankai University, Tianjin

来源：

Huagong Jinzhan/Chemical Industry and Engineering Progress | 2023年 / 42卷 / 05期

关键词：

egg white; electrodialysis with ultrafiltration membrane (EDUF); lysozyme (LYZ); protein; purification; separation;

D O I：

10.16085/j.issn.1000-6613.2022-1293

中图分类号：

学科分类号：

摘要：

Lysozyme (LYZ) is a natural active protein with stable chemical properties, non-toxic and antibacterial function. Lysozyme with high purity and high activity has great value in the fields of food and medicine. In this paper, a novel electrodialysis with ultrafiltration membrane (EDUF) technology was used to directly separate LYZ from egg white based on the difference in charge and molecular weight between ovalbumin (OVA) and LYZ under specific pH conditions. The effects of ultrafiltration membrane material and the solution flow rates in feed and recovery compartments on the separation performance of EDUF were investigated. Results showed that the recovery rate and purity of LYZ could respectively reached 21.05% and 100% by using PVDF100Ⅰmembrane when the operating voltage was 5.0V and the solution flow rates in feed and recovery compartments were both 50mL/min. The final transport rate of LYZ was 0.21g/(m2·h) and energy consumption per unit product was 2.30kW·h/g under the tested conditions. This study demonstrates that EDUF, as an unique electro-driven membrane separation technology, has remarkable characteristics with its high selectivity. EDUF technology has good application prospects in the field of high-efficient separation of active proteins. © 2023 Chemical Industry Press. All rights reserved.

引用

页码：2262 / 2271

页数：9

共 21 条

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