Engineering Embryonic Stem Cell Microenvironments for Tailored Cellular Differentiation

被引：0
作者：
Huang, Chenyu [1 ,2 ]
Melerzanov, Alexander [3 ]
Du, Yanan [4 ]
机构：
[1] Department of Plastic, Reconstructive and Aesthetic Surgery, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing
[2] Department of Plastic Surgery, Meitan General Hospital, Beijing
[3] Cellular and Molecular Technologies Laboratory, MIPT, Dolgoprudny
[4] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing
来源：
Journal of Nanotechnology in Engineering and Medicine | 2015年 / 6卷 / 04期
关键词：
directed differentiation; embryonic stem cells; Hydrogel; microenvironment engineering;
D O I：
10.1115/1.4033193
中图分类号：
学科分类号：
摘要：
The rapid progress of embryonic stem cell (ESCs) research offers great promise for drug discovery, tissue engineering, and regenerative medicine. However, a major limitation in translation of ESCs technology to pharmaceutical and clinical applications is how to induce their differentiation into tailored lineage commitment with satisfactory efficiency. Many studies indicate that this lineage commitment is precisely controlled by the ESC microenvironment in vivo. Engineering and biomaterial-based approaches to recreate a biomimetic cellular microenvironment provide valuable strategies for directing ESCs differentiation to specific lineages in vitro. In this review, we summarize and examine the recent advances in application of engineering and biomaterial-based approaches to control ESC differentiation. We focus on physical strategies (e.g., geometrical constraint, mechanical stimulation, extracellular matrix (ECM) stiffness, and topography) and biochemical approaches (e.g., genetic engineering, soluble bioactive factors, coculture, and synthetic small molecules), and highlight the three-dimensional (3D) hydrogel-based microenvironment for directed ESC differentiation. Finally, future perspectives in ESCs engineering are provided for the subsequent advancement of this promising research direction. © 2016 by ASME.
引用
收藏
相关论文
共 72 条
[71]  
Elder B.D., Eleswarapu S.V., Athanasiou K.A., Extraction techniques for the decellularization of tissue engineered articular cartilage constructs, Biomaterials, 30, 22, pp. 3749-3756, (2009)
[72]  
Cortiella J., Niles J., Cantu A., Brettler A., Pham A., Vargas G., Winston S., Wang J., Walls S., Nichols J.E., Influence of acellular natural lung matrix on murine embryonic stem cell differentiation and tissue formation, Tissue Eng., Part A, 16, 8, pp. 2565-2580, (2010)
12345678