Engineering Embryonic Stem Cell Microenvironments for Tailored Cellular Differentiation

被引：0
作者：
Huang, Chenyu [1 ,2 ]
Melerzanov, Alexander [3 ]
Du, Yanan [4 ]
机构：
[1] Department of Plastic, Reconstructive and Aesthetic Surgery, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing
[2] Department of Plastic Surgery, Meitan General Hospital, Beijing
[3] Cellular and Molecular Technologies Laboratory, MIPT, Dolgoprudny
[4] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing
来源：
Journal of Nanotechnology in Engineering and Medicine | 2015年 / 6卷 / 04期
关键词：
directed differentiation; embryonic stem cells; Hydrogel; microenvironment engineering;
D O I：
10.1115/1.4033193
中图分类号：
学科分类号：
摘要：
The rapid progress of embryonic stem cell (ESCs) research offers great promise for drug discovery, tissue engineering, and regenerative medicine. However, a major limitation in translation of ESCs technology to pharmaceutical and clinical applications is how to induce their differentiation into tailored lineage commitment with satisfactory efficiency. Many studies indicate that this lineage commitment is precisely controlled by the ESC microenvironment in vivo. Engineering and biomaterial-based approaches to recreate a biomimetic cellular microenvironment provide valuable strategies for directing ESCs differentiation to specific lineages in vitro. In this review, we summarize and examine the recent advances in application of engineering and biomaterial-based approaches to control ESC differentiation. We focus on physical strategies (e.g., geometrical constraint, mechanical stimulation, extracellular matrix (ECM) stiffness, and topography) and biochemical approaches (e.g., genetic engineering, soluble bioactive factors, coculture, and synthetic small molecules), and highlight the three-dimensional (3D) hydrogel-based microenvironment for directed ESC differentiation. Finally, future perspectives in ESCs engineering are provided for the subsequent advancement of this promising research direction. © 2016 by ASME.
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共 72 条
[1]  
Evans M.J., Kaufman M.H., Establishment in culture of pluripotent cells from mouse embryos, Nature, 292, 5819, pp. 154-156, (1981)
[2]  
Martin G.R., Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells, Proc. Natl. Acad. Sci. U.S.A., 78, 12, pp. 7634-7638, (1981)
[3]  
Murry C.E., Keller G., Differentiation of embryonic stem cells to clinically relevant populations: Lessons from embryonic development, Cell, 132, 4, pp. 661-680, (2008)
[4]  
Edalat F., Bae H., Manoucheri S., Cha J.M., Khademhosseini A., Engineering approaches toward deconstructing and controlling the stem cell environment, Ann. Biomed. Eng., 40, 6, pp. 1301-1315, (2012)
[5]  
Stojkovic M., Lako M., Strachan T., Murdoch A., Derivation, growth and applications of human embryonic stem cells, Reproduction, 128, 3, pp. 259-267, (2004)
[6]  
Keller G., Embryonic stem cell differentiation: Emergence of a new era in biology and medicine, Genes Dev., 19, 10, pp. 1129-1155, (2005)
[7]  
Toh Y.C., Blagovic K., Voldman J., Advancing stem cell research with microtechnologies: Opportunities and challenges, Integr. Biol. (Camb), 2, 7-8, pp. 305-325, (2010)
[8]  
Zhang H., Dai S., Bi J., Liu K.K., Biomimetic three-dimensional microenvironment for controlling stem cell fate, Interface Focus, 1, 5, pp. 792-803, (2011)
[9]  
Xu Z., Wang J., Du Y., Recent advances in embryonic stem cell engineering toward tailored lineage differentiation, Emerging Trends in Cell and Gene Therapy, pp. 33-54, (2013)
[10]  
Burdick J.A., Vunjak-Novakovic G., Engineered microenvironments for controlled stem cell differentiation, Tissue Eng., Part A, 15, 2, pp. 205-219, (2009)
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