Genome-wide Identification and Expression Analysis of the Rice BXL Gene Family

被引：0

作者：

Wu, Hao ^{[1
]}

Dong, Wei-Feng ^{[1
]}

He, Zi-Tian ^{[1
]}

Li, Yan-Xiao ^{[1
]}

Xie, Hui ^{[2
]}

Sun, Ming-Zhe ^{[1
,3
]}

Shen, Yang ^{[1
,4
]}

Sun, Xiao-Li ^{[1
]}

机构：

[1] Crop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing

[2] National Japonica Rice Engineering and Technology Research Center, Tianjin

[3] Beidahuang Kenfeng Seed Industry Co., Ltd., Harbin

[4] Heilongjiang Bachihe Technology Co., Ltd., Jiamusi

来源：

Biotechnology Bulletin | 2025年 / 41卷 / 06期

关键词：

abiotic stress; grain size; hormone response; rice; β-D-xylosidase;

D O I：

10.13560/j.cnki.biotech.bull.1985.2024-1215

中图分类号：

学科分类号：

摘要：

【Objective】BXL (ȕ-D-xylosidase) belongs to the third family of glycoside hydrolases, which catalyzes the degradation of xylan in the cell wall, and plays a key role in regulating plant growth and development as well as abiotic stress response. Evolutionary and expression analysis of the OsBXL gene family will facilitate further exploration of their functions in rice.【Method】Bioinformatic technologies were used for analyzing the phylogenetic relationships, replication events, gene structure, conserved motifs, and tissue expression profiles of OsBXLs, and fluorescence quantitative PCR was used to investigate the expression pattern of OsBXLs under abiotic stress and hormone treatments.【Result】There are 10 members in rice BXL gene family, which can be divided into three subfamilies, with Group III specific to monocots. The gene structures and structural domains of OsBXLs within each group showed a high similarity. Gene location analysis indicated that OsBXL family members were unevenly distributed on 4 chromosomes, with OsBXL5/6/7/8 on chromosome 4 in a gene cluster. Transcriptome data revealed that OsBXL1/2/3/4/8 showed high expressions in a majority of detected tissues/organs, while OsBXL5/6/7/10 were expressed at low levels. Notably, the expressions of OsBXL7/10 were significantly high at the certain stage during the maturation of young inflorescence and seed. Moreover, three haplotypes of OsBXL7 were identified in the natural population, and significant differences in grain length, width, and thousand-grain weight were observed among different haplotypes. Fluorescence quantitative PCR revealed different expression patterns of OsBXLs under drought, saline-alkali, abscisic acid and methyl jasmonate treatments. 【Conclusion】 The OsBXL gene family is highly conserved, OsBXL7 is possibly involved in regulating rice grain size, while OsBXL1/3 simultaneously respond to salt-alkali stress and methyl jasmonate treatment. Together, these results provide a key guide for future functional analyses of OsBXL genes in rice. © 2025 The Editorial Office of Biotechnology Bulletin. All rights reserved.

引用

页码：87 / 98

页数：11

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