Structure identification and free radical scavenging activity of polysaccharide from fruit bodies of leucopaxillus giganteus

被引：0

作者：

Bao Y. ^{[1
]}

Ma Y. ^{[1
]}

Li Y. ^{[1
,2
]}

Zhang Y. ^{[1
]}

Wu Y. ^{[1
,2
]}

Liu C. ^{[1
,2
]}

机构：

[1] College of Life and Environmental Science, Minzu University of China, Beijing

[2] Beijing Engineering Research Center of Food Environment and Public Health, Beijing

来源：

Shipin Kexue/Food Science | 2016年 / 37卷 / 06期

关键词：

Free radicals; Isolation and purification; Leucopaxillus giganteus; Polysaccharide; Structure;

D O I：

10.7506/spkx1002-6630-201606012

中图分类号：

学科分类号：

摘要：

Crude polysaccharide was extracted from the fruit bodies of Leucopaxillus giganteus by water extraction and alcohol precipitation and was purified through sequential steps: acidic ethanol fractionation, Sepharose CL-4B and Sephadex G-100 gel filtration chromatography to homogeneity as confirmed by high performance liquid chromatography (HPLC). The purified polysaccharide was designated as DBZG4-b and its molecular weight was measured by HPLC. Moreover, the structure of DBZG4-b was elucidated by means of gas chromatography, paper chromatography, infrared (IR) spectroscopy, gas chromatography-mass spectrometry after methylation, and nuclear magnetic resonance (NMR). Results showed that the average molecular weight of the homogeneous polysaccharide, DBZG4-b, was 6864 D. Its main chains were composed of Glc and Gal, and both of them were conjugated to 1→6 glycoside bond; Glc was substituted at 3-O while Gal was substituted at 4-O. The side chain was composed of(1→3)-Ara,(1→3)-Glc, and(1→4)-Gal. The terminal residue was made up of Ara, Xyl, Rha, Man, Glc and Gal. Furthermore, DBZG4-b had significant scavenging potential against hydrolxyl and DPPH free radicals. © 2016, China Food Publishing Company. All right reserved.

引用

页码：71 / 76

页数：5

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