Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

被引：0

作者：

Gunashree, B.S. ^{[1
]}

Venkateswaran, G. ^{[1
]}

机构：

[1] Department of Food Microbiology, Central Food Technological Research Institute, Mysore,570 020, India

来源：

Journal of Food Science and Technology | 2015年 / 52卷 / 07期

关键词：

Dextran - Ion exchange - Column chromatography - Ion exchange resins - Enzymes - Aspergillus - Sodium chloride - Biochemistry;

D O I：

暂无

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学科分类号：

摘要：

Phytase, that is extensively used as a feed additive is capable of hydrolyzing phytic acid, an antinutrient found in about 60–80 % of all the plant commodities. This enzyme improves the bioavailability of essential minerals such as Ca2+, Mg2+, P, Zn2+, Fe3+, that are bound to phytic acid. An extracellular phytase from a local fungal isolate, Aspergillus niger CFR 335 was purified to homogeneity through a three-step column chromatography using DEAE-Sephadex anion exchanger. An active fraction of the enzyme was obtained with NaCl gradient of 2.5 M in DEAE Sephadex column. The enzyme was purified up to 16 fold with a yield of 28.5 %. Substrate specificity studies revealed a highest specific activity of 32.6 ± 3.1 U/mg for sodium phytate with the Km value of 0.08 ± 0.1 mM. The molecular weight of the enzyme was 66 kDa with an optimum temperature of 30 °C and pH 4.5. Up to 80 % of the activity was retained even after storing the enzyme for 6 months at 4 °C. © 2014, Association of Food Scientists & Technologists (India).

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页码：4558 / 4564