Determination of tuberculosis biomarker CFP-10 in serum by ultra-performance liquid chromatography-tandem mass spectrometry

被引：0

作者：

Fang, Mei ^{[1
]}

Xu, Wenfang ^{[2
]}

Wang, Jiaojiao ^{[1
]}

Wu, Xiaodan ^{[1
]}

Jin, Faxiang ^{[2
]}

Li, Shimin ^{[1
,3
]}

机构：

[1] Analysis Center of Agrobiology and Environment Sciences of Zhejiang University Laboratory, Hangzhou,310058, China

[2] Department of Clinical Laboratory, Affiliated Hospital of Shaoxing University, Shaoxing,312000, China

[3] Zeta Precision (Hangzhou) Biotechnology Co, Hangzhou,310051, China

来源：

Chinese Journal of Analysis Laboratory | 2025年 / 44卷 / 04期

关键词：

Biomarkers - Blood - High performance liquid chromatography - High pressure liquid chromatography;

D O I：

10.13595/j.cnki.issn1000-0720.2023122204

中图分类号：

学科分类号：

摘要：

An ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method was developed to determine the tuberculosis （TB） biomarker CFP-10 in human serum. After protein precipitation and trypsin digestion， solid-phase extraction （SPE） was used to enrich the specific peptide produced during enzymatic digestion of culture filtrate protein 10（ CFP-10）. The separation of the target analyte was performed on a Luna Omega PS-C18 column. The specific CFP-10 peptide was quantified under positive electrospray ion electrospray ionization （ESI+） and multiple reaction monitoring （MRM） mode. The results showed that the calibration curve was linear in the range of 3.0‒100 ng/mL for CFP-10， with the correlation coefficient of r2=0.9906. The limit of detection （LOD） and limit of quantification （LOQ） for CFP-10 were 1.0 and 3.0 ng/mL， respectively. The spiked recoveries ranged from 89.8% to 108.1%， with the intra-day and inter-day relative standard deviations （RSDs） both lower than 10%. Serum CFP-10 levels in TB and non-TB control cases were compared， and the results showed that serum CFP-10 was significantly lower in TB control than in non-TB cases. The proposed method is suitable for the rapid determination of CFP-10 in clinical human serum samples. © 2025, Youke Publishing Co.,Ltd. All rights reserved.

引用

页码：539 / 543