Terminal deoxynucleotidyl transferase: Properties and applications

被引：0

作者：

Zhang, Chengjie ^{[1
]}

Subthain, Hizar ^{[1
]}

Guo, Fei ^{[1
]}

Fang, Peng ^{[1
]}

Zheng, Shanmin

Shen, Mengzhe ^{[3
]}

Jiang, Xianger ^{[3
]}

Gao, Zhengquan ^{[2
]}

Meng, Chunxiao ^{[2
]}

Li, Shengying ^{[4
]}

Du, Lei ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China

[2] Binzhou Med Univ, Sch Pharm, Yantai 264003, Shandong, Peoples R China

[3] BGI Res, Shenzhen 518083, Peoples R China

[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China

来源：

ENGINEERING MICROBIOLOGY | 2025年 / 5卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Terminal deoxynucleotidyl transferase; Template-independent DNA synthesis; Synthetic biology; Enzyme engineering; DNA-POLYMERASE-BETA; V(D)J RECOMBINATION; FLUORESCENCE DETECTION; CRYSTAL-STRUCTURES; MOLECULAR-BIOLOGY; N-REGIONS; MECHANISM; INFORMATION; DISTINCT; GENES;

D O I：

10.1016/j.engmic.2024.100179; 10.1016/j.engmic.2024.100179

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3 ' terminal of a DNA chain in atemplate-independent manner. Owing to their remarkable DNA synthesis activity, TdTs have promoted the development of numerous nucleic acid-based methods, tools, and associated applications, attracting broad interest from both academia and industry. This review summarizes and discusses the recent research on TdTs, including their biochemical characteristics, enzyme engineering, and practical applications. New insights and perspectives on the future development of TdTs are provided.

引用

页数：13

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