Preparation and characterization of surface molecularly imprinted polymers for selective adsorption of ethopabate

被引：0

作者：

Zhang, Lamei ^{[1
]}

Li, Daomin ^{[1
]}

Li, Zhaozhou ^{[1
]}

Liu, Erwei ^{[1
]}

Li, Songbiao ^{[1
]}

Cao, Li ^{[1
]}

Gao, Hongli ^{[1
]}

Wang, Fang ^{[1
]}

Hou, Yuze ^{[1
]}

机构：

[1] College of Food and Bioengineering, Henan University of Science and Technology, Luoyang,471003, China

来源：

Shipin Kexue/Food Science | 2016年 / 37卷 / 04期

关键词：

Monomers - Dissociation - Ethylene glycol - Molecular modeling - Polymerization - Silica gel - Biosynthesis - Crosslinking - Ethylene;

D O I：

10.7506/spkx1002-6630-201604041

中图分类号：

学科分类号：

摘要：

The novel functional monomer N,O-bismethacryloyl ethanolamine (NOBE) was synthesized and used to prepare molecularly imprinted polymers (ETP-MIPs) for adsorbing ethopabate by surface molecular imprinting technique using silica gel as the support matrix and ethylene glycol dimethacrylate (EGDMA) as the crosslinking agent. Three polymerization conditions including molar ratio of template to functional monomer, molar ratio of template to crosslinking agent and porogen type were optimized using an orthogonal array design. The type of porogen was the most important factor, followed by the ratio of template to functional monomer and ratio of template to crosslinking agent. The best imprinting efficiency was observed for molecularly imprinted polymers with a molar ratio of ETP to NOBE to EGDMA of 1:2:20 using acetonitrile as the porogen. The Scatchard analysis indicated that there were two classes of binding sites in ETP-MIPs; the equilibrium dissociation constant (Kd) and apparent maximum binding capacity (Qmax) for the high affinity sites and low affinity sites were calculated as follows: Kd1=1.608 μg/mL, Qmax1=1.101 μg/mg; and Kd2=0.109 μg/mL, Qmax2=0.172 μg/mg, respectively. © 2016, China Food Publishing Company. All right reserved.

引用

页码：226 / 232