Construction of Lactobacillus delbrueckii subsp. bulgaricus thyA-null strains

被引：0

作者：

Li, Chen ^{[1
]}

Guo, Xin ^{[1
]}

Lu, Haiqiang ^{[1
]}

Tian, Jingjing ^{[2
]}

Gu, Xinxi ^{[1
]}

Tian, Hongtao ^{[1
]}

Luo, Yunbo ^{[3
]}

机构：

[1] College of Food Science and Technology, Agricultural University of Hebei, Baoding

[2] College of Food Sciences, Beijing University of Agriculture, Beijing

[3] College of Food Science and Nutritional Engineering, China Agricultural University, Beijing

来源：

Journal of Chinese Institute of Food Science and Technology | 2015年 / 15卷 / 11期

关键词：

Insertional mutagenesis; Lactobacillus delbrueckii subsp. bulgaricus; pGhost9:ISS1; ThyA gene;

D O I：

10.16429/j.1009-7848.2015.11.004

中图分类号：

学科分类号：

摘要：

Objective: Using the insertion sequence ISS1 construction mutants, in order to isolate the Lactobacillus delbrueckii subsp. bulgaricus thyA -null strains. Methods: After ISS1 replicative transposition, the chromosome contains duplicated ISS1 sequences flanking pGhost, Lactobacillus delbrueckii subsp. bulgaricus is efficient mutagenesis. The mutants were cultivated on MRS supplement with deoxythymidine(50 μg·mL-1) and TMP (1 to 500 μg·mL-1, depending on the strain). After about 33 generations of cultivation, each culture broth was spread on MRS agar plates containing de-oxythymidine. Those single-colonies isolates of each strain were examined by growth on SA agar with and without added deoxythymidine. Results: Achieving the thyA-null strains with the insetion of ISS1 and the mutants are stable. © 2015, Chinese Institute of Food Science and Technology. All right reserved.

引用

页码：23 / 28

页数：5

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