Uridine and uridine nucleotides as novel regulators of NUCB2/nesfatin-1 neuron activation: an immunohistochemical study

被引：0

作者：

Hasanoglu Akbulut, N. ^{[1
,2
]}

Topal, G. ^{[1
]}

Koc, C. ^{[3
]}

Coskan, N. ^{[1
]}

Salman, B. ^{[3
]}

Cansev, M. ^{[3
]}

Eyigor, O. ^{[1
]}

机构：

[1] Bursa Uludag Univ, Fac Med, Dept Histol & Embryol, Bursa, Turkiye

[2] Canakkale Onsekiz Mart Univ, Fac Med, Dept Histol & Embryol, Canakkale, Turkiye

[3] Bursa Uludag Univ, Fac Med, Dept Pharmacol, Bursa, Turkiye

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2025年 / 163卷 / 01期

关键词：

Uridine; Uridine nucleotides; NUCB2; Nesfatin-1; Neuronal activation; Hypothalamic nuclei; FOOD-INTAKE; P2; RECEPTORS; NESFATIN-1; INCREASES; EXPRESSION; ANXIETY; INSULIN; PROTEIN; IMMUNOREACTIVITY; SUPPLEMENTATION;

D O I：

10.1007/s00418-025-02404-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nesfatin-1, identified as an anorexigenic neuropeptide in the hypothalamus, is activated by glutamatergic agonists and innervated by excitatory and inhibitory neurons. The uridine nucleotide uridine diphosphate was recently identified as a novel regulator of feeding-related neurons. However, the activating effects of uridine and uridine nucleotides on anorexigenic NUCB2/nesfatin-1 neurons are unknown. In this study, the activating effects of intracerebroventricularly administered uridine or uridine nucleotides (UMP, UDP, UTP) on NUCB2/nesfatin-1 neurons localized in the supraoptic (SON), paraventricular (PVN), and periventricular (PeV) nuclei were determined using c-Fos immunohistochemistry. Results were evaluated as the percentage of the ratio of c-Fos-expressing (activated) NUCB2/nesfatin-1 neurons to all NUCB2/nesfatin-1 neurons. It was determined that centrally administered uridine, UMP, and UTP activated a statistically significant number of NUCB2/nesfatin-1 neurons in the SON, PVN, and PeV, compared with the saline group (p < 0.05). At the same time, the slight increase in the neuronal activation seen following UDP application was not found to be significant. The results of this study show that NUCB2/nesfatin-1 neurons respond to uridine and uridine nucleotides in the form of neuronal activation, possibly through pyrimidinergic neurotransmission.

引用

页数：11

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