Regulation of astaxanthin accumulation in Phaffia rhodozyma under titanium dioxide and rapeseed oil stress by amino acid metabolism

被引：0

作者：

Liu, Meizhen ^{[1
]}

Han, Tiantian ^{[1
]}

Luo, Lu ^{[2
]}

Zhang, Ying ^{[1
]}

Yuan, Gaofeng ^{[1
]}

Fang, Xubo ^{[3
]}

Dong, Keyu ^{[1
]}

Zhang, Jing ^{[1
]}

Chen, Xiaoe ^{[1
]}

机构：

[1] Zhejiang Ocean Univ, Coll Food & Pharm, Zhoushan, Peoples R China

[2] Zhejiang Ocean Univ, Sch Naval Architecture & Maritime, Zhoushan, Peoples R China

[3] Zhejiang Int Maritime Coll, Zhoushan, Peoples R China

来源：

FOOD CHEMISTRY | 2025年 / 492卷

关键词：

Natural astaxanthin; Dual stress conditions; Metabolite profiling; Amino acid; MECHANISMS; CAROTENOIDS; GLUTAMATE; REVEALS; GROWTH; ROLES;

D O I：

10.1016/j.foodchem.2025.145406

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study aimed to identify effective strategies for the sustainable production of natural astaxanthin by Phaffia rhodozyma. The combination of titanium dioxide (TiO2) and rapeseed oil boosted astaxanthin production in P. rhodozyma PR106 to 47.31 mg/L, a 6.24-fold increase compared to the control. Meanwhile, the levels of phytoene, lycopene, and beta-carotene decreased by 16.8 %, 17.4 %, and 4.0 %, respectively. Notably, TiO2 and rapeseed oil enhanced the activity of superoxide dismutase and catalase, lowering reactive oxygen species levels. The mechanisms underlying the enhanced astaxanthin production in PR106 involve significant changes in intracellular purine metabolism, ABC transporters, glycolysis, the TCA cycle, and amino acid metabolism induced by TiO2 and rapeseed oil stress. Finally, this study confirmed that amino acid metabolism (particularly involving succinic acid and valine) played a crucial regulatory role in the biosynthesis of astaxanthin. This research provides a robust theoretical foundation for the industrial development of natural astaxanthin.

引用

页数：13

共 67 条

[1] ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis [J].

Ali, Eunus S. ;

Sahu, Umakant ;

Villa, Elodie ;

O'Hara, Brendan P. ;

Gao, Peng ;

Beaudet, Cynthia ;

Wood, Antony W. ;

Asara, John M. ;

Ben-Sahra, Issam .

MOLECULAR CELL, 2020, 78 (06) :1178-+

[2] Roles of glycine betaine and proline in improving plant abiotic stress resistance [J].

Ashraf, M. ;

Foolad, M. R. .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2007, 59 (02) :206-216

[3] Biosynthesis of Astaxanthin as a Main Carotenoid in the Heterobasidiomycetous Yeast Xanthophyllomyces dendrorhous [J].

Barredo, Jose L. ;

Garcia-Estrada, Carlos ;

Kosalkova, Katarina ;

Barreiro, Carlos .

JOURNAL OF FUNGI, 2017, 3 (03)

[4] A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast [J].

Bharadwaj, Prashant ;

Martins, Ralph .

NEURAL REGENERATION RESEARCH, 2020, 15 (10) :1931-1936

[5] Regulation of Amino Acid Transport in Saccharomyces cerevisiae [J].

Bianchi, Frans ;

van't Klooster, Joury S. ;

Ruiz, Stephanie J. ;

Poolman, Bert .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2019, 83 (04)

[6] Glutamate, at the interface between amino acid and carbohydrate metabolism [J].

Brosnan, JT .

JOURNAL OF NUTRITION, 2000, 130 (04) :988S-990S

[7] Transcription factors and ABC transporters: from pleiotropic drug resistance to cellular signaling in yeast [J].

Buechel, Evan R. ;

Pinkett, Heather W. .

FEBS LETTERS, 2020, 594 (23) :3943-3964

[8] Energy metabolism analysis of exogenous glutamate on promoting co-accumulation of astaxanthin yield and biomass in Phaffia rhodozyma D3 [J].

Cai, Chunyu ;

Xu, Na ;

Feng, Jiale ;

Zhang, Jiahua ;

Zhao, Qianxi ;

Liu, Huimin ;

Nan, Bo ;

Li, Xia ;

Wang, Yuhua .

BIORESOURCE TECHNOLOGY, 2024, 402

[9] Thermal stability of astaxanthin in oils for its use in fish food technology [J].

Calvo, N. S. ;

Reynoso, C. M. ;

Resnik, S. ;

Cortes-Jacinto, E. ;

Collins, P. .

ANIMAL FEED SCIENCE AND TECHNOLOGY, 2020, 270

[10]

Capelli B., 2013, NUTRAfoods, V12, P145

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