Different Circulating Endothelial Microvesicle Subtype Signature in Subacute and Chronic Spinal Cord Injury

被引：0
作者：
Park, Andrew J. [1 ,2 ]
Fandl, Hannah K. [3 ]
Garcia, Vinicius P. [3 ]
Berry, Auburn R. [3 ]
Wegerson, Kendra N. [3 ]
Ostrander, Emily I. [3 ]
Cardenas, Hannah L. [3 ]
Desouza, Noah M. [3 ]
Greiner, Jared J. [3 ]
Stauffer, Brian [4 ,5 ]
Desouza, Christopher A. [3 ]
机构：
[1] Craig Hosp, Rocky Mt Reg Spinal Injury Syst, Englewood, CO USA
[2] Univ Colorado, Dept Phys Med & Rehabil, Aurora, CO USA
[3] Univ Colorado, Dept Integrat Physiol, Integrat Vasc Biol Lab, Boulder, CO USA
[4] Univ Colorado, Sch Med, Div Cardiol, Anschutz Med Campus, Aurora, CO USA
[5] Denver Hlth Med Ctr, Div Cardiol, Denver, CO USA
来源：
TOPICS IN SPINAL CORD INJURY REHABILITATION | 2025年 / 31卷 / 02期
基金：
美国国家卫生研究院;
关键词：
cardiovascular disease; endothelial microvesicles; spinal cord injury; OXIDATIVE STRESS; PHYSICAL-ACTIVITY; VASCULAR FUNCTION; MICROPARTICLES; ACTIVATION; RISK; APOPTOSIS; EXPRESSION; MICRORNA; DISEASE;
D O I：
10.46292/sci24-00068
中图分类号：
R49 [康复医学];
学科分类号：
100215 ;
摘要：
Objectives: The aim of this study was to determine whether circulating concentrations of activation- and apoptosis-derived endothelial cell-derived microvesicles (EMVs) differ between adults after subacute (time since injury <= 6 months) and chronic (time since injury >12 months) spinal cord injury (SCI). Methods: Peripheral blood was collected from 43 adults (age range 18-71 years): 12 non-injured adults (9 male/3 female), 16 adults with subacute cervical and high thoracic (C2-T3) motor complete injuries (13 male/3 female; time since injury 1-3 months), and 15 adults with chronic cervical and high thoracic (C1-T2) motor complete injuries (14 male/1 female; time since injury 12-52 months). EMVs were defined by markers of endothelial origin either by activation (CD62e(+)) or apoptosis (CD31(+)/CD42b(-)) by flow cytometry. Activation-derived but not apoptosis-derived EMVs were significantly higher (P < .05) in adults with chronic SCI (median [IQR], 139 [83-181] EMVs/mu L) compared with adults with subacute SCI (median [IQR], 99 [83-104] EMVs/mu L) and non-injured adults (median [IQR], 74 [51-104] EMVs/mu L). In contrast, apoptosis-derived but not activation-derived EMVs were significantly higher (P < .05) in adults with subacute SCI (mean +/- SD, 77 +/- 17 EMVs/mu L) compared with adults with chronic SCI (mean +/- SD, 55 +/- 19 EMVs/mu L) and non-injured adults (mean +/- SD, 52 +/- 25 EMVs/mu L). Differential expression of circulating EMVs in adults with SCI during the subacute and chronic phase of injury may represent a biomarker of the vascular environment associated with each condition. Our findings suggest that the vascular phenotype is markedly different in subacute compared with the chronic SCI and provide insight into endothelial function after SCI.
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页码：29 / 38
页数：10
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