Liver organoids: Current advances and future applications for hepatology

被引:0
作者
Kim, Yohan [1 ,2 ,3 ]
Kang, Minseok [4 ]
Mamo, Michael Girma [4 ,5 ]
Adisasmita, Michael [4 ,5 ]
Huch, Meritxell [6 ]
Choi, Dongho [4 ,5 ,7 ,8 ]
机构
[1] Sungkyunkwan Univ, Dept MetaBioHealth, Suwon, South Korea
[2] Sungkyunkwan Univ, Dept Biopharmaceut Convergence, Suwon, South Korea
[3] Sungkyunkwan Univ, Biomed Inst Convergence SKKU, Suwon, South Korea
[4] Hanyang Univ, Coll Med, Dept Surg, 222 Wangsimni Ro, Seoul 04763, South Korea
[5] Hanyang Univ, Res Inst Regenerat Med & Stem Cells, Seoul, South Korea
[6] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany
[7] Hanyang Univ, Hanyang Inst Biosci & Biotechnol, Seoul, South Korea
[8] Hanyang Univ, Dept HY KIST Bioconvergence, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
Organoids; Liver organoids; Organoid applications; Regenerative medicine; Hepatology; SINUSOIDAL ENDOTHELIAL-CELLS; FUNCTIONAL HUMAN LIVER; IN-VITRO; STEM-CELLS; PRIMARY HEPATOCYTES; DRUG-METABOLISM; CULTURE; TOXICITY; MODELS; GENERATION;
D O I
10.3350/cmh.2024.1040
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The creation of self-organizing liver organoids represents a significant, although modest, step toward addressing the ongoing organ shortage crisis in allogeneic liver transplantation. However, researchers have recognized that achieving a fully functional whole liver remains a distant goal, and the original ambition of organoid-based liver generation has been temporarily put on hold. Instead, liver organoids have revolutionized the field of hepatology, extending their influence into various domains of precision and molecular medicine. These 3D cultures, capable of replicating key features of human liver function and pathology, have opened new avenues for human-relevant disease modeling, CRISPR gene editing, and high-throughput drug screening that animal models cannot accomplish. Moreover, advancements in creating more complex systems have led to the development of multicellular assembloids, dynamic organoid-on-chip systems, and 3D bioprinting technologies. These innovations enable detailed modeling of liver microenvironments and complex tissue interactions. Progress in regenerative medicine and transplantation applications continues to evolve and strives to overcome the obstacles of biocompatibility and tumorigenecity. In this review, we examine the current state of liver organoid research by offering insights into where the field currently stands, and the pivotal developments that are shaping its future. (Clin Mol Hepatol 2025;31(Suppl):S327-S348)
引用
收藏
页码:S327 / S348
页数:364
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