The Effect of Dental Bleaching on Nanohybrid Composite Surface Roughness: A Comparative In Vitro Study of SEM and Profilometry

Background: This study aimed to evaluate the effect of in-office bleaching with 38% hydrogen peroxide (HP) on the surface roughness of a nanohybrid composite resin by comparing two measurement techniques: Scanning Electron Microscopy (SEM) and profilometry. Methods: Sixty composite specimens of identical shade and thickness were prepared, light-cured, and polished following the manufacturer's guidelines. These samples were divided into six groups based on the applied surface treatments: group 1: fresh composite (the control group), group 2: old composite, group 3: bleached fresh composite, group 4: bleached old composite, group 5: old repolished composite, and group 6: old repolished bleached composite. Surface roughness was measured using profilometry and SEM. Results: Pearson correlation analysis revealed a moderately significant linear relationship (r = 0.548, p < 0.001) between the surface roughness measurements obtained using SEM and the profilometer, indicating that both methods provide comparable results. A comparison of most groups showed significant differences (p < 0.001), highlighting the increased surface roughness observed after bleaching both fresh and aged composites. Conclusions: Bleaching increased the surface roughness of nanohybrid composites. It might be better to use SEM and a profilometer together to obtain a more comprehensive understanding of the surface characteristics.