Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE

Background: Helminthic excretory secretory (E/S) antigens gained considerable interest due to their role in stimulating host-parasite interactions and as potential candidate for vaccine development. Objective: To quantify and characterize H. contortus E/S antigens aiming for its use as an effective control measure. Material and Methods: Adult worms were collected from the abomasa of slaughtered goats. Fresh and highly motile adult worms were transferred to RPMI 1640 culture medium containing antibiotics. The E/S antigen was procured through standard protocols and concentration of proteins was measured. Characterization of ES antigen was subjected to SDS-PAGE and estimation of protein concentration. Results: The triturate contained proteins with a concentration of 0.836 mg/ml. Three bands of proteins appeared on the gel with molecular weights of 65, 48 and 30 kDa. Conclusion: Further characterization of E/S antigens will be helpful in developing effective control measures against the worms in general and haemonchosis specifically.