Adipokines level in plasma, hypothalamus, ovaries and adipose tissue of rats with polycystic ovary syndrome

被引：0

作者：

Pich, Karolina ^{[1
,2
]}

Pietron, Klaudia ^{[1
]}

Szlaga, Agata ^{[3
]}

Billert, Maria ^{[4
]}

Skrzypski, Marek ^{[4
]}

Pawlicki, Piotr ^{[5
]}

Kotula-Balak, Malgorzata ^{[6
]}

Dupont, Joelle ^{[7
]}

Blasiak, Anna ^{[3
]}

Rak, Agnieszka ^{[1
]}

机构：

[1] Jagiellonian Univ Krakow, Inst Zool & Biomed Res, Lab Physiol & Toxicol Reprod, Krakow, Poland

[2] Jagiellonian Univ, Doctoral Sch Exact & Nat Sci, Krakow, Poland

[3] Jagiellonian Univ, Inst Zool & Biomed Res, Dept Neurophysiol & Chronobiol, Krakow, Poland

[4] Poznan Univ Life Sci, Dept Anim Physiol Biochem & Biostruct, Poznan, Poland

[5] Agr Univ Krakow, Ctr Expt & Innovat Med, Krakow, Poland

[6] Agr Univ Krakow, Univ Ctr Vet Med JU UA, Dept Anim Anat & Preclin Sci, Krakow, Poland

[7] INRAE, Physiol Reprod & Comportements, Nouzilly, France

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2025年 / 50卷 / 05期

关键词：

PCOS; Adipokines; Hypothalamus; Ovary; Periovarian adipose tissue; Rat; SERUM CHEMERIN LEVELS; GENE-EXPRESSION; INSULIN-RESISTANCE; APELIN LEVELS; NUTRITIONAL-STATUS; MENSTRUAL-CYCLE; NORMAL-WEIGHT; ADIPONECTIN; WOMEN; VASPIN;

D O I：

10.1016/j.rbmo.2024.104693

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Research question: Do the levels of adipokines (adiponectin, apelin, chemerin and vaspin) in plasma, hypothalamus, ovaries and periovarian adipose tissue differ during polycystic ovarian syndrome (PCOS)? Design: The PCOS was induced in rats by oral administration of non-steroidal aromatase inhibitor letrozole. To determine the plasma levels of adiponectin, apelin, chemerin and vaspin enzyme-linked immunosorbent assays were carried out. To assess the expression (gene and protein) and immunolocalization of these adipokines and their receptors, namely Adipor1 and Adipor2 for adiponectin, Aplnr for apelin, Ccrl2, Cmklr1 and Gpr1 for chemerin and Grp78 for vaspin in the hypothalamus, real-time polymerase chain reaction, and Western blot and immunohistochemistry were used to analyse ovaries and periovarian adipose tissue respectively. Results: In PCOS, the plasma level of adiponectin decreased (P = 0.0003), whereas apelin, chemerin and vaspin increased (P < 0.0479). Moreover, PCOS modulates the expression of adipokines and their receptors in the hypothalamus, ovaries and periovarian adipose tissue compared with healthy rats (P < 0.487). Conclusions: A strong relationship was found between PCOS and adipokines, which suggests that adipokines may be a biomarker of PCOS.

引用

页数：18

共 102 条

[1] Relationship among plasma adipokines, insulin and androgens level as well as biochemical glycemic and lipidemic markers with incidence of PCOS in women with normal BMI [J].