Rapid Detection of Salmonella and Listeria monocytogenes in Fresh Beef Using Loop-Mediated Isothermal Amplification

被引：0

作者：

Wang, Xiaoran ^{[1
]}

Jia, Kaixiang ^{[1
]}

Ran, Jinrong ^{[1
]}

Du, Yangyang ^{[1
]}

Yu, Xiaoying ^{[1
]}

Cao, Xuefeng ^{[1
]}

Peng, Lianci ^{[1
]}

Chen, Weifeng ^{[2
]}

Fang, Rendong ^{[1
]}

Li, Zhiwei ^{[1
]}

机构：

[1] Southwest Univ, Coll Vet Med, Joint Int Res Lab Anim Hlth & Anim Food Safety, Chongqing 400715, Peoples R China

[2] Henan Univ Anim Husb & Econ, Sch Food & Bioengn, Zhengzhou, Peoples R China

来源：

FOODBORNE PATHOGENS AND DISEASE | 2025年

关键词：

loop-mediated isothermal amplification (LAMP); Salmonella; Listeria monocytogenes; rapid detection; fresh beef; LAMP; ENTERICA; CHAIN; FOOD;

D O I：

10.1089/fpd.2025.0018

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study developed and optimized loop-mediated isothermal amplification (LAMP) assays for rapid detection of Salmonella and L. monocytogenes in fresh beef. LAMP primers targeting the invA gene of Salmonella and hly gene of L. monocytogenes were used. Reaction conditions including temperature, dNTP concentration, Mg2+ concentration, primer ratio, and Bst DNA polymerase amount were optimized for each pathogen. Differences were observed between the two pathogens in optimal temperature, Mg2+ concentration, and Bst DNA polymerase requirements, highlighting the importance of pathogen-specific optimization. The optimized assays demonstrated high sensitivity with detection limits of 120 fg/mu L for Salmonella and 130 fg/mu L for L. monocytogenes, achieving detection within 40 and 60 min, respectively. Specificity tests confirmed both assays were highly specific for their target pathogens in fresh beef samples with no cross-reactivity observed. Addition of hydroxynaphthol blue enabled simple visual detection of positive results, eliminating the need for specialized equipment. The developed LAMP assays offer rapid, sensitive, and specific detection of these important foodborne pathogens.

引用

页数：8

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