Exploration of comprehensive two-dimensional liquid chromatography : Theoretical aspects and practical considerations-A tutorial

被引：0
作者：
Arena, Katia [1 ]
Cacciola, Francesco [1 ]
Vinci, Roberto Lagana
Dugo, Paola [1 ,2 ]
Mondello, Luigi [1 ,2 ]
机构：
[1] Univ Messina, Messina Inst Technol MeIT, Former Vet Sch, Dept Chem Biol Pharmaceut & Environm Sci, I-98168 Messina, Italy
[2] Univ Messina, Former Vet Sch, Chromaleont srl, Dept Chem Biol Pharmaceut & Environm Sci, I-98168 Messina, Italy
来源：
JOURNAL OF CHROMATOGRAPHY OPEN | 2025年 / 7卷
关键词：
LCxLC; Multidimensional chromatography; Complex samples; method optimization; EFFECTIVE PEAK-CAPACITY; REVERSED-PHASE SEPARATIONS; TANDEM MASS-SPECTROMETRY; ORTHOGONALITY; COLUMN; RESOLUTION; SYSTEMS; MODULATION; IDENTIFICATION; 2ND-DIMENSION;
D O I：
10.1016/j.jcoa.2025.100222
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Comprehensive two-dimensional liquid chromatography (LCxLC) is a valuable tool for the analysis of very complex samples. LCxLC, which involves the coupling of two or more orthogonal separation systems, is a potential alternative to the conventional liquid-chromatography (1D-LC) approach. The profits of such a technique, compared to the 1D-LC approach, are the increased resolving power along with significant reduced ion enhancement or suppression phenomena in the MS source. This tutorial aims to provide detailed information on theoretical and practical aspects of the LCxLC approach with a particular focus on column combination, method optimization and separation performance. In addition, future perspectives and new trends are highlighted.
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页数：16
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