Diagnostic Utility of Bronchoalveolar Lavage Flow Cytometric Leukocyte Profiling in Interstitial Lung Disease and Infection

被引：0

作者：

Novoa-Bolivar, Erika M. ^{[1
]}

Ros, Jose A. ^{[2
]}

Perez-Fernandez, Sonia ^{[3
]}

Campillo, Jose A. ^{[1
]}

Lopez-Hernandez, Ruth ^{[1
]}

Gonzalez-Lopez, Rosana ^{[1
]}

Ruiz-Lorente, Inmaculada ^{[1
]}

Otalora-Alcaraz, Almudena ^{[1
]}

Ortuno-Hernandez, Cristina ^{[1
]}

Gimeno, Lourdes ^{[1
]}

Ceballos-Francisco, Diana ^{[1
]}

Muro, Manuel ^{[1
]}

Solana-Martinez, Elena ^{[2
]}

Martinez-Camblor, Pablo ^{[4
]}

Minguela, Alfredo ^{[1
]}

机构：

[1] Virgen Arrixaca Univ Clin Hosp HCUVA, Biomed Res Inst Murcia Pascual Parrilla IMIB, Immunol Serv, Murcia 30120, Spain

[2] Virgen Arrixaca Univ Clin Hosp HCUVA, Biomed Res Inst Murcia Pascual Parrilla IMIB, Pneumol Serv, Murcia 30120, Spain

[3] Univ Oviedo, Dept Stat & Operat Res & Math Didact, Asturias 33007, Spain

[4] Geisel Sch Med Dartmouth, Dept Biomed Data Sci, 7 Lebanon St,Suite 309,Hinman Box 7261, Hanover, NH 03755 USA

来源：

BIOMOLECULES | 2025年 / 15卷 / 04期

关键词：

interstitial lung disease; lung infection; flow cytometry; bronchoalveolar lavage; leukocyte profile; lung fibrosis; FLUID; CLASSIFICATION; RATIOS; BLOOD;

D O I：

10.3390/biom15040597

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interstitial lung diseases (ILD) represent a diverse group of disorders that primarily affect the pulmonary interstitium and, less commonly, involve the alveolar and vascular epithelium. Overlapping clinical, radiological and histopathological features make proper classification difficult, requiring multiple complementary methodologies, including flow cytometry of bronchoalveolar lavages (BAL). This retrospective study analyzed BAL flow cytometry data from 1074 real-life patients, quantifying alveolar macrophages, CD4/CD8 lymphocytes, neutrophils, eosinophils, and CD1a+ Langerhans cells, with the aim of evaluating its diagnostic utility in ILD and pulmonary infection. Clustering and logistic regression analyses identified seven distinct leukocyte profiles: lymphocytic (associated with hypersensitivity pneumonitis, cryptogenic organizing pneumonia, and lymphocytic interstitial pneumonia), sarcoidosis, macrophagic (including nonspecific interstitial pneumonia, desquamative interstitial pneumonitis, pneumoconiosis, and unclassifiable ILD), neutrophilic (including usual interstitial pneumonia, respiratory bronchiolitis ILD, and acute interstitial pneumonia), infectious diseases, eosinophilic ILD, and Langerhans cell histiocytosis. The estimated leukocyte profiles were associated with different overall survival (OS) outcomes. Neutrophilic profiles, both infectious and non-infectious, correlated with poorer OS, particularly in patients without pulmonary fibrosis. Furthermore, corticosteroids and other immunosuppressive therapies did not show significant OS differences across leukocyte profiles. Although the gold standard in BAL cytology continues to be cytopathology, these results support BAL flow cytometry as a rapid and reliable complementary tool to aid in the classification of interstitial lung diseases based on immune cell profiles, providing valuable predictive information and contributing to personalized therapeutic approaches.

引用

页数：14

共 33 条

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