Biofilm characterisation of the maize rot-causing pathogen, Fusarium verticillioides

被引：0

作者：

Peremore, Chizne ^{[1
,2
]}

van 't Hof, Cairin ^{[1
]}

Nkosi, Cebo-LeNkosi ^{[1
]}

Tshiyoyo, Kadima ^{[1
]}

Ratsoma, Francinah M. ^{[1
,2
]}

Kola, Wisely ^{[1
,2
]}

Malgas, Samkelo ^{[1
]}

Santana, Quentin ^{[2
,3
]}

Wingfield, Brenda ^{[1
,2
]}

Steenkamp, Emma T. ^{[1
,2
]}

Motaung, Thabiso E. ^{[1
,2
]}

机构：

[1] Univ Pretoria, Dept Biochem Genet & Microbiol, Pretoria, South Africa

[2] Univ Pretoria, Forestry & Agr Biotechnol Inst, Pretoria, South Africa

[3] Agr Res Council, Biotechnol Platform, Pretoria, South Africa

来源：

BIOFOULING | 2025年 / 41卷 / 06期

基金：

芬兰科学院; 新加坡国家研究基金会;

关键词：

Biofilm; Fusarium verticillioides; extracellular polymeric substances; extracellular DNA; virulence factors; EXTRACELLULAR DNA; CHITOSAN; ACETYLATION; CHITIN; POLYSACCHARIDE; MYCOTOXINS; RESISTANCE; CARBON;

D O I：

10.1080/08927014.2025.2512097

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biofilm formation was investigated in a maize rot-causing pathogen, Fusarium verticillioides. This work revealed that in vitro cultures produce structured, adherent communities with a dense extracellular matrix (ECM) surrounding hyphae that makes up the biomass of a matured biofilm. Pellicle containing exopolysaccharide had a hydrodynamic diameter of 4.19 nm and a low viscosity (0.022 dl/g). The exopolysaccharide was composed of amino sugars and unordered, facilitating stability through complexation with the anionic eDNA. Biofilm formation varied over different pH and temperature values, emphasising its role in promoting adaption, survival, and persistence in F. verticillioides, potentially contributing to its pathogenicity in maize. Collectively, the results provide valuable insights into biofilm structure and stress resistance in this fungus, and will serve as a foundation for future studies incorporating in planta infection systems.

引用

页码：586 / 605

页数：20

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