Gene editing using CRISPR-Cas9 technology: potential implications in assisted reproduction

被引:0
作者
Sahin, Gizem Nur [1 ]
Seli, Emre [1 ]
机构
[1] Yale Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT USA
关键词
Cas9; CRISPR; gene editing; CRISPR/CAS9; TECHNOLOGY; DNA METHYLATION; GENOME; CAS9; ACTIVATION; TRANSCRIPTION; SPECIFICITY; EXPRESSION; EFFICIENCY; FREQUENCY;
D O I
10.1097/GCO.0000000000001022
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Purpose of reviewThis article reviews the mechanisms, advancements, and potential implications of clustered regularly interspaced short palindromic repeats-associated (CRISPR-Cas) gene editing technology, with a specific focus on its applications in reproductive biology and assisted reproduction. It aims to explore the benefits and challenges of integrating this revolutionary technology into clinical and research settings.Recent findingsCRISPR-Cas9 is a transformative tool for precise genome editing, enabling targeted modifications through mechanisms like nonhomologous end joining (NHEJ) and homology-directed repair (HDR). Innovations such as Cas9 nickase and dCas9 systems have improved specificity and expanded applications, including gene activation, repression, and epigenetic modifications. In reproductive research, CRISPR has facilitated gene function studies, corrected genetic mutations in animal models, and demonstrated potential in addressing human infertility and hereditary disorders. Emerging applications include mitochondrial genome editing, population control of disease vectors via gene drives, and detailed analyses of epigenetic mechanisms.SummaryCRISPR-Cas9 technology has revolutionized genetic engineering by enabling precise genome modifications. This article discusses its mechanisms, focusing on the repair pathways (NHEJ and HDR) and methods to mitigate off-target effects. In reproductive biology, CRISPR has advanced our understanding of fertility genes, allowed corrections of hereditary mutations, and opened avenues for novel therapeutic strategies. While its clinical application in human-assisted reproduction faces ethical and safety challenges, ongoing innovations hold promise for broader biomedical applications.
引用
收藏
页码:141 / 148
页数:8
相关论文
共 72 条
[51]   Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions [J].
Morita, Sumiyo ;
Noguchi, Hirofumi ;
Horii, Takuro ;
Nakabayashi, Kazuhiko ;
Kimura, Mika ;
Okamura, Kohji ;
Sakai, Atsuhiko ;
Nakashitna, Hideyuki ;
Hata, Kenichiro ;
Nakashima, Kinichi ;
Hatada, Izuho .
NATURE BIOTECHNOLOGY, 2016, 34 (10) :1060-1065
[52]   High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity [J].
Pattanayak, Vikram ;
Lin, Steven ;
Guilinger, John P. ;
Ma, Enbo ;
Doudna, Jennifer A. ;
Liu, David R. .
NATURE BIOTECHNOLOGY, 2013, 31 (09) :839-+
[53]   Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression [J].
Qi, Lei S. ;
Larson, Matthew H. ;
Gilbert, Luke A. ;
Doudna, Jennifer A. ;
Weissman, Jonathan S. ;
Arkin, Adam P. ;
Lim, Wendell A. .
CELL, 2013, 152 (05) :1173-1183
[54]   Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity [J].
Ran, F. Ann ;
Hsu, Patrick D. ;
Lin, Chie-Yu ;
Gootenberg, Jonathan S. ;
Konermann, Silvana ;
Trevino, Alexandro E. ;
Scott, David A. ;
Inoue, Azusa ;
Matoba, Shogo ;
Zhang, Yi ;
Zhang, Feng .
CELL, 2013, 154 (06) :1380-1389
[55]   Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling [J].
Ravichandran, Mirunalini ;
Maddalo, Danilo .
FRONTIERS IN GENETICS, 2023, 14
[56]   Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos [J].
Reyes, Alvaro Plaza ;
Lanner, Fredrik .
DEVELOPMENT, 2017, 144 (01) :3-7
[57]   Efficient high-precision homology-directed repair-dependent genome editing by HDRobust [J].
Riesenberg, Stephan ;
Kanis, Philipp ;
Macak, Dominik ;
Wollny, Damian ;
Duesterhoeft, Dorothee ;
Kowalewski, Johannes ;
Helmbrecht, Nelly ;
Maricic, Tomislav ;
Paeaebo, Svante .
NATURE METHODS, 2023, 20 (09) :1388-+
[58]  
Royal Swedish Academy of Sciences, 2020, Nobel Prize in Chemistry 2020 Press Release
[59]   Prime editing - an update on the field [J].
Scholefield, Janine ;
Harrison, Patrick T. .
GENE THERAPY, 2021, 28 (7-8) :396-401
[60]   Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells [J].
Shalem, Ophir ;
Sanjana, Neville E. ;
Hartenian, Ella ;
Shi, Xi ;
Scott, David A. ;
Mikkelsen, Tarjei S. ;
Heckl, Dirk ;
Ebert, Benjamin L. ;
Root, David E. ;
Doench, John G. ;
Zhang, Feng .
SCIENCE, 2014, 343 (6166) :84-87