Hammerhead Ribozymes: Structural Insights, Catalytic Mechanisms, and Cutting-Edge Applications in Synthetic Biology

被引：0

作者：

Wang, Liangliang ^{[1
,2
]}

Liu, Yan ^{[1
,3
]}

Xian, Xuemin ^{[1
]}

Zhang, Haitao ^{[1
]}

机构：

[1] Guangdong Med Univ, Dept Biochem & Mol Biol, Zhanjiang 524023, Peoples R China

[2] Lanzhou Jiaotong Univ, Sch Biol & Pharmaceut Engn, Lanzhou 730070, Peoples R China

[3] Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2025年 / 26卷 / 12期

关键词：

hammerhead ribozymes; RNA catalysis; gene regulation; antiviral therapy; biosensing; SELF-CLEAVING RIBOZYMES; GENE-EXPRESSION; ACTIVE-SITE; CRYSTAL-STRUCTURE; RNA CATALYSIS; CLEAVAGE; RIBOSWITCHES; SELECTION; REQUIREMENTS; REPLICATION;

D O I：

10.3390/ijms26125624

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hammerhead ribozymes are a class of small RNA molecules with catalytic activity. Their compact size, high catalytic efficiency, structural simplicity, and modular design flexibility make them ideal tools for RNA manipulation and gene regulation. In recent years, these ribozymes have demonstrated tremendous application potential across diverse fields, including gene regulation, disease therapy, and biosensing, significantly advancing related research. This article provides a comprehensive review of recent progress in hammerhead ribozyme research within synthetic biology, thoroughly examines the current challenges, and outlines future development directions, aiming to offer valuable perspectives and insights for their biomedical applications.

引用

页数：14

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