Repurposed Drugs to Enhance the Therapeutic Potential of Oligodendrocyte Precursor Cells Derived from Adult Rat Adipose Tissue

被引：0

作者：

Pascual-Guerra, J. ^{[1
,2
]}

Torres-Rico, M. ^{[2
]}

Marin-Rodriguez, B. ^{[2
]}

Arasmou-Idrovo, M. S. ^{[2
]}

Garcia, A. G. ^{[2
,3
]}

Rodriguez-Navarro, J. A. ^{[1
,4
]}

Paino, C. L. ^{[1
]}

机构：

[1] Hosp Univ Ramon y Cajal, Serv Neurobiol Invest, IRYCIS, Madrid 28034, Spain

[2] Fdn Teofilo Hernando, Madrid 28049, Spain

[3] Univ Autonoma Madrid, Fac Med, Dept Farmacol, Madrid 28023, Spain

[4] Univ Complutense Madrid, Inst Univ Invest Neuroquim IUIN, Fac Farm, Dept Bioquim & Biol Mol, Madrid 28040, Spain

来源：

CELLS | 2025年 / 14卷 / 07期

关键词：

drug repurposing; oligodendrocytes precursor cells; myelin; lentiviral transduction; MUSCARINIC RECEPTOR SUBTYPES; PROGENITOR CELLS; MONOCLONAL-ANTIBODIES; EFFICIENT GENERATION; DIFFERENTIATION; PROLIFERATION; MIGRATION; REMYELINATION; RECOVERY; SYSTEM;

D O I：

10.3390/cells14070533

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Failure in the proliferation, recruitment, mobilization, and/or differentiation of oligodendrocyte precursor cells (OPCs) impedes remyelination in central nervous system (CNS) demyelinating diseases. Our group has recently achieved the generation of functional oligodendroglia through direct lineage conversion by expressing Sox10, Olig2, and Zfp536 genes in adult rat adipose tissue-derived stromal cells. The present study aimed to determine whether various repurposed drugs or molecules could enhance the myelinating capacities of these induced OPCs (iOPCs). We report that kainate, benztropine, miconazole, clobetasol, and baclofen promote in vitro iOPCs migration, differentiation, and ensheathing abilities through mechanisms similar to those observed in rat neural stem cell-derived OPCs. This research supports the potential use of iOPCs as they provide an alternative and reliable cell source for testing the effects of in vitro promyelinating repurposed drugs and for assessing the molecular and cellular mechanisms involved in therapeutic strategies for demyelinating diseases.

引用

页数：29

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