Ginsenoside Rb1 Alleviates DSS-Induced Ulcerative Colitis by Protecting the Intestinal Barrier Through the Signal Network of VDR, PPARγ and NF-κB

被引:4
作者
Zhou, Yi [1 ]
Xiong, Xinyu [1 ]
Cheng, Zhe [1 ]
Chen, Zekai [1 ]
Wu, Shizhen [2 ]
Yu, Yan [3 ]
Liu, Yujin [4 ]
Chen, Guang [1 ]
Li, Lingli [5 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Integrated Tradit Chinese & Western Med, Tongji Med Coll, Wuhan 430030, Peoples R China
[2] Hubei Univ Chinese Med, Coll Acupuncture & Bone Injury, Wuhan 430061, Peoples R China
[3] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Gastroenterol, Tongji Med Coll, Wuhan 430030, Peoples R China
[4] Huazhong Univ Sci & Technol, Union Hosp, Dept Integrated Tradit Chinese & Western Med, Tongji Med Coll, Wuhan 430022, Peoples R China
[5] Wuhan Fourth Hosp, Dept Tradit Chinese Med, Wuhan 430033, Peoples R China
关键词
ginsenoside Rb1; ulcerative colitis; intestinal barrier; vitamin D receptor; nuclear factor-kappa B; VITAMIN-D-RECEPTOR; EPITHELIAL-CELLS; PANAX-GINSENG;
D O I
10.2147/DDDT.S481769
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Purpose: Ginseng (Panax ginseng Meyer) is an herbal medicine used in traditional Chinese medicine (TCM), has the effects of treating colitis and other diseases. Ginsenoside Rb1 (GRb1), a major component of ginseng, modulates autoimmunity and metabolism. However, the mechanism underlying GRb1 treatment of ulcerative colitis (UC) has not yet been elucidated. UC is a refractory inflammatory bowel disease (IBD) with a high recurrence rate, and researches on new drugs for UC have been in the spotlight for a long time. Methods: Mice with DSS-induced UC were treated with GRb1 or 0.9% saline for 10 days. Colon tissue of UC mice was collected to detect the levels of intestinal inflammatory cytokines and integrity of the intestinal barrier. RNA-seq and network pharmacology were used to predict the therapeutic targets of GRb1 during UC treatment. Results: GRb1 treatment alleviated intestinal inflammation and improved intestinal barrier dysfunction in UC mice. Specifically, GRb1 downregulated the levels of pro-inflammatory cytokines such as TNF-alpha and IL-6, while upregulating the level of the anti-inflammatory cytokine IL-10. Additionally, GRb1 treatment increased the levels of tight junction proteins including ZO-1, Occludin, and E-cadherin, which are crucial for maintaining intestinal barrier integrity. Further analyses using RNA-seq and network pharmacology suggested that these effects might involve the regulation of GRb1 in the signal transduction network of VDR, PPAR gamma, and NF kappa B. Conclusion: The study demonstrated that GRb1 effectively alleviated UC by modulating intestinal inflammation and protecting the integrity of the intestinal barrier through the signal transduction network of VDR, PPAR gamma, and NF-kappa B.
引用
收藏
页码:4825 / 4838
页数:14
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