Advanced single-cell RNA sequencing in tumor immunology

被引：0

作者：

Liu, Yilong ^{[1
]}

Yu, Xiyuan ^{[2
]}

Han, Zhipeng ^{[2
]}

Wei, Qiuyang ^{[1
]}

Niu, Qi ^{[2
]}

Yang, Chaoyong ^{[3
]}

Li, Jie P. ^{[1
]}

Guo, Zijian ^{[1
]}

Zhang, Huimin ^{[3
]}

机构：

[1] Nanjing Univ, Chem & Biomed Innovat Ctr ChemBIC, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Nanjing, Peoples R China

[2] Xi An Jiao Tong Univ, Inst Analyt Chem & Instrument Life Sci, Key Lab Biomed Informat Engn, Minist Educ,Sch Life Sci & Technol, Xian, Shaanxi, Peoples R China

[3] Xiamen Univ, Coll Chem & Chem Engn, Innovat Lab Sci & Technol Energy Mat Fujian Prov I, State Key Lab Vaccines Infect Dis,Xiang An Biomed, Xiamen 361102, Fujian, Peoples R China

来源：

INTERDISCIPLINARY MEDICINE | 2025年

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

immunotherapy; single-cell RNA sequencing; T cell receptor; tumor immunology; tumor microenvironment; INFILTRATING T-CELLS; SEQ; REVEALS; LANDSCAPE; IMMUNOTHERAPY; RESPONSES; ATLAS;

D O I：

10.1002/INMD.20250060

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Cancer immunotherapy is an innovative treatment approach that leverages the immune system to combat tumors, demonstrating significant therapeutic potential. In the past few years, single-cell RNA sequencing (scRNA-seq) has made significant progress in the field of cancer immunotherapy, enabling us to understand the complexity of anti-tumor immune processes with unprecedented depth and precision, thereby facilitating the design of more effective immunotherapy strategies. This review aims to summarize the recent applications of advanced scRNA-seq technologies in tumor immunology. First, we outline the most representative scRNA-seq technologies with different technical principles, with a particular focus on single-cell T cell receptor sequencing. Next, we describe how scRNA-seq technology is applied to identify the cellular composition and phenotypes within the tumor microenvironment for the construction of immune cell atlas, and uncover key cell types and molecular mechanisms underlying treatment responses for developing novel immunotherapies. Finally, we address the current challenges and future prospects of scRNA-seq technology in tumor immunology.

引用

页数：16

共 64 条

[31] Bispecific dendritic-T cell engager potentiates anti-tumor immunity [J].

Itai, Yuval Shapir ;

Barboy, Oren ;

Salomon, Ran ;

Bercovich, Akhiad ;

Xie, Ken ;

Winter, Eitan ;

Shami, Tamar ;

Porat, Ziv ;

Erez, Neta ;

Tanay, Amos ;

Amit, Ido ;

Dahan, Rony .

CELL, 2024, 187 (02) :375-389.e18

[32] Time-resolved single-cell transcriptomics defines immune trajectories in glioblastoma [J].

Kirschenbaum, Daniel ;

Xie, Ken ;

Ingelfinger, Florian ;

Katzenelenbogen, Yonatan ;

Abadie, Kathleen ;

Look, Thomas ;

Sheban, Fadi ;

San Phan, Truong ;

Li, Baoguo ;

Zwicky, Pascale ;

Yofe, Ido ;

David, Eyal ;

Mazuz, Kfir ;

Hou, Jinchao ;

Chen, Yun ;

Shaim, Hila ;

Shanley, Mayra ;

Becker, Soeren ;

Qian, Jiawen ;

Colonna, Marco ;

Ginhoux, Florian ;

Rezvani, Katayoun ;

Theis, Fabian J. ;

Yosef, Nir ;

Weiss, Tobias ;

Weiner, Assaf ;

Amit, Ido .

CELL, 2024, 187 (01) :149-165.e23

[33] Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells [J].

Klein, Allon M. ;

Mazutis, Linas ;

Akartuna, Ilke ;

Tallapragada, Naren ;

Veres, Adrian ;

Li, Victor ;

Peshkin, Leonid ;

Weitz, David A. ;

Kirschner, Marc W. .

CELL, 2015, 161 (05) :1187-1201

[34] UDA-seq: universal droplet microfluidics-based combinatorial indexing for massive-scale multimodal single-cell sequencing [J].

Li, Yun ;

Huang, Zheng ;

Xu, Lubin ;

Fan, Yanling ;

Ping, Jun ;

Li, Guochao ;

Chen, Yanjie ;

Yu, Chengwei ;

Wang, Qifei ;

Song, Turun ;

Lin, Tao ;

Liu, Mengmeng ;

Xu, Yangqing ;

Ai, Na ;

Meng, Xini ;

Qiao, Qin ;

Ji, Hongbin ;

Qin, Zhen ;

Jin, Shuo ;

Jiang, Nan ;

Wang, Minxian ;

Shu, Shaokun ;

Zhang, Feng ;

Zhang, Weiqi ;

Liu, Guang-Hui ;

Chen, Limeng ;

Jiang, Lan .

NATURE METHODS, 2025, 22 (06) :1199-1212

[35] Microfluidic single-cell transcriptomics: moving towards multimodal and spatiotemporal omics [J].

Lin, Shichao ;

Liu, Yilong ;

Zhang, Mingxia ;

Xu, Xing ;

Chen, Yingwen ;

Zhang, Huimin ;

Yang, Chaoyong .

LAB ON A CHIP, 2021, 21 (20) :3829-3849

[36] Identification of a tumour immune barrier in the HCC microenvironment that determines the efficacy of immunotherapy [J].

Liu, Yao ;

Xun, Zhenzhen ;

Ma, Kun ;

Liang, Shuhang ;

Li, Xianying ;

Zhou, Shuo ;

Sun, Linmao ;

Liu, Yufeng ;

Du, Yanhua ;

Guo, Xinyu ;

Cui, Tianming ;

Zhou, Huanran ;

Wang, Jizhou ;

Yin, Dalong ;

Song, Ruipeng ;

Zhang, Shugeng ;

Cai, Wei ;

Meng, Fanzheng ;

Guo, Hongrui ;

Zhang, Bo ;

Yang, Di ;

Bao, Rujuan ;

Hu, Qingsong ;

Wang, Jiabei ;

Ye, Youqiong ;

Liu, Lianxin .

JOURNAL OF HEPATOLOGY, 2023, 78 (04) :770-782

[37] Neoadjuvant PARPi or chemotherapy in ovarian cancer informs targeting effector Treg cells for homologous-recombination-deficient tumors [J].

Luo, Yikai ;

Xia, Yu ;

Liu, Dan ;

Li, Xiong ;

Li, Huayi ;

Liu, Jiahao ;

Zhou, Dongchen ;

Dong, Yu ;

Li, Xin ;

Qian, Yiyu ;

Xu, Cheng ;

Tao, Kangjia ;

Li, Guannan ;

Pan, Wen ;

Zhong, Qing ;

Liu, Xingzhe ;

Xu, Sen ;

Wang, Zhi ;

Liu, Ronghua ;

Zhang, Wei ;

Shan, Wanying ;

Fang, Tian ;

Wang, Siyuan ;

Peng, Zikun ;

Jin, Ping ;

Jin, Ning ;

Shi, Shennan ;

Chen, Yuxin ;

Wang, Mengjie ;

Jiao, Xiaofei ;

Luo, Mengshi ;

Gong, Wenjian ;

Wang, Ya ;

Yao, Yue ;

Zhao, Yi ;

Huang, Xinlin ;

Ji, Xuwo ;

He, Zhaoren ;

Zhao, Guangnian ;

Liu, Rong ;

Wu, Mingfu ;

Chen, Gang ;

Hong, Li ;

Ma, Ding ;

Fang, Yong ;

Liang, Han ;

Gao, Qinglei .

CELL, 2024, 187 (18) :4905-4925

[38] Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets [J].

Macosko, Evan Z. ;

Basu, Anindita ;

Satija, Rahul ;

Nemesh, James ;

Shekhar, Karthik ;

Goldman, Melissa ;

Tirosh, Itay ;

Bialas, Allison R. ;

Kamitaki, Nolan ;

Martersteck, Emily M. ;

Trombetta, John J. ;

Weitz, David A. ;

Sanes, Joshua R. ;

Shalek, Alex K. ;

Regev, Aviv ;

McCarroll, Steven A. .

CELL, 2015, 161 (05) :1202-1214

[39] Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis [J].

Mathewson, Nathan D. ;

Ashenberg, Orr ;

Tirosh, Itay ;

Gritsch, Simon ;

Perez, Elizabeth M. ;

Marx, Sascha ;

Jerby-Arnon, Livnat ;

Chanoch-Myers, Rony ;

Hara, Toshiro ;

Richman, Alyssa R. ;

Ito, Yoshinaga ;

Pyrdol, Jason ;

Friedrich, Mirco ;

Schumann, Kathrin ;

Poitras, Michael J. ;

Gokhale, Prafulla C. ;

Castro, L. Nicolas Gonzalez ;

Shore, Marni E. ;

Hebert, Christine M. ;

Shaw, Brian ;

Cahill, Heather L. ;

Drummond, Matthew ;

Zhang, Wubing ;

Olawoyin, Olamide ;

Wakimoto, Hiroaki ;

Rozenblatt-Rosen, Orit ;

Brastianos, Priscilla K. ;

Liu, X. Shirley ;

Jones, Pamela S. ;

Cahill, Daniel P. ;

Frosch, Matthew P. ;

Louis, David N. ;

Freeman, Gordon J. ;

Ligon, Keith L. ;

Marson, Alexander ;

Chiocca, E. Antonio ;

Reardon, David A. ;

Regev, Aviv ;

Suva, Mario L. ;

Wucherpfennig, Kai W. .

CELL, 2021, 184 (05) :1281-+

[40] Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR [J].

Munson, Daniel J. ;

Egelston, Colt A. ;

Chiotti, Kami E. ;

Parra, Zuly E. ;

Bruno, Tullia C. ;

Moore, Brandon L. ;

Nakano, Taizo A. ;

Simons, Diana L. ;

Jimenez, Grecia ;

Yim, John H. ;

Rozanov, Dmitri V. ;

Falta, Michael T. ;

Fontenot, Andrew P. ;

Reynolds, Paul R. ;

Leach, Sonia M. ;

Borges, Virginia F. ;

Kappler, John W. ;

Spellman, Paul T. ;

Lee, Peter P. ;

Slansky, Jill E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (29) :8272-8277

← 1 2 3 4 5 6 7 →