Construction of a Macrophage-Tropic Subtype C HIV-1-mGreenLantern Reporter Virus for Studies on HIV-1 Replication and the Impact of Methamphetamine

被引:0
作者
Mofed, Dina [1 ,2 ]
Mandarino, Angelo [1 ,2 ]
Wu, Xuhong [3 ]
Lang, Yuekun [1 ,2 ,4 ]
Gowripalan, Anjali [1 ,2 ,5 ]
Kalpana, Ganjam V. [3 ]
Prasad, Vinayaka R. [1 ,2 ]
机构
[1] Albert Einstein Coll Med, Dept Microbiol, Bronx, NY 10461 USA
[2] Albert Einstein Coll Med, Dept Immunol, Bronx, NY 10461 USA
[3] Albert Einstein Coll Med, Dept Genet, Bronx, NY 10461 USA
[4] Coll Vet Med, Dept Vet Pathobiol, Columbia, MO 65211 USA
[5] Australian Natl Univ, John Curtin Sch Med Res, Div Immunol & Infect Dis, Canberra 2601, Australia
来源
VIRUSES-BASEL | 2024年 / 16卷 / 12期
关键词
replication competent HIV-1 reporter; subtype C HIV-1; CCL2; methamphetamine; NF-KAPPA-B; FULL-LENGTH; TYPE-1; PROTEIN; CELLS; TRANSCRIPTION; INFECTION; ENHANCER; FITNESS; FUSION;
D O I
10.3390/v16121859
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
HIV-1 subtype C viruses are responsible for 50% of global HIV burden. However, nearly all currently available reporter viruses widely used in HIV research are based on subtype B. We constructed and characterized a replication-competent HIV-1 subtype C reporter virus expressing mGreenLantern. mGreenLantern sequences were inserted in-frame with Nef ATG in HIV-1IndieC1. As controls, we employed HIV-1IndieC1, HIV-1ADA, and HIV-1NLAD8-GFP-Nef viruses. HIV-1IndieC1-mGreenLantern (HIV-1IndieC1-mGL) exhibited characteristics of the parental HIV-1IndieC1 virus, including its infectivity in TZMbl reporter cells and replication competence in macrophages. To further characterize HIV-1IndieC1-mGL virus, we tested its responsiveness to CCL2 levels, a characteristic feature of subtype B HIV-1 that is missing in subtype C. CCL2 immunodepletion inhibited the production of HIV-1ADA and HIV-1NLAD8-GFP-Nef as expected, but not that of HIV-1IndieC1-mGL, as previously reported. We also tested the effect of methamphetamine, as its effect is mediated by NF-kB and since subtype C viruses carry an additional copy of NF-kB. We found that methamphetamine increased the replication of all viruses tested in macrophages; however, its effect was much more robust for HIV-1IndieC1 and HIV-1IndieC1-mGL. Our studies established that HIV-1IndieC1-mGL retains all the characteristics of the parental HIV-1IndieC1 and can be a useful tool for HIV-1 subtype C investigations.
引用
收藏
页数:14
相关论文
共 38 条
[1]   CCL2 mobilizes ALIX to facilitate Gag-p6 mediated HIV-1 virion release [J].
Ajasin, David O. ;
Rao, Vasudev R. ;
Wu, Xuhong ;
Ramasamy, Santhamani ;
Pujato, Mario ;
Ruiz, Arthur P. ;
Fiser, Andras ;
Bresnick, Anne R. ;
Kalpana, Ganjam, V ;
Prasad, Vinayaka R. .
ELIFE, 2019, 8
[2]   Multiple NF-κB Sites in HIV-1 Subtype C Long Terminal Repeat Confer Superior Magnitude of Transcription and Thereby the Enhanced Viral Predominance [J].
Bachu, Mahesh ;
Yalla, Swarupa ;
Asokan, Mangaiarkarasi ;
Verma, Anjali ;
Neogi, Ujjwal ;
Sharma, Shilpee ;
Murali, Rajesh V. ;
Mukthey, Anil Babu ;
Bhatt, Raghavendra ;
Chatterjee, Snehajyoti ;
Rajan, Roshan Elizabeth ;
Cheedarla, Narayana ;
Yadavalli, Venkat S. ;
Mahadevan, Anita ;
Shankar, Susarla K. ;
Rajagopalan, Nirmala ;
Shet, Anita ;
Saravanan, Shanmugam ;
Balakrishnan, Pachamuthu ;
Solomon, Suniti ;
Vajpayee, Madhu ;
Satish, Kadappa Shivappa ;
Kundu, Tapas K. ;
Jeang, Kuan-Teh ;
Ranga, Udaykumar .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (53) :44714-44735
[3]   Comparing the ex vivo fitness of CCR5-tropic human immunodeficiency virus type 1 isolates of subtypes B and C [J].
Ball, SC ;
Abraha, A ;
Collins, KR ;
Marozsan, AJ ;
Baird, H ;
Quiñones-Mateu, ME ;
Penn-Nicholson, A ;
Murray, M ;
Richard, N ;
Lobritz, M ;
Zimmerman, PA ;
Kawamura, T ;
Blauvelt, A ;
Arts, EJ .
JOURNAL OF VIROLOGY, 2003, 77 (02) :1021-1038
[4]   An additional NF-κB site allows HIV-1 subtype C to evade restriction by nuclear PYHIN proteins [J].
Bosso, Matteo ;
Sturzel, Christina M. ;
Kmiec, Dorota ;
Badarinarayan, Smitha Srinivasachar ;
Braun, Elisabeth ;
Ito, Jumpei ;
Sato, Kei ;
Hahn, Beatrice H. ;
Sparrer, Konstantin M. J. ;
Sauter, Daniel ;
Kirchhoff, Frank .
CELL REPORTS, 2021, 36 (12)
[5]   mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging [J].
Campbell, Benjamin C. ;
Nabel, Elisa M. ;
Murdock, Mitchell H. ;
Lao-Peregrin, Cristina ;
Tsoulfas, Pantelis ;
Blackmore, Murray G. ;
Lee, Francis S. ;
Liston, Conor ;
Morishita, Hirofumi ;
Petsko, Gregory A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (48) :30710-30721
[6]   Labeling HIV-1 virions with two fluorescent proteins allows identification of virions that have productively entered the target cell [J].
Campbell, Edward M. ;
Perez, Omar ;
Melar, Marta ;
Hope, Thomas J. .
VIROLOGY, 2007, 360 (02) :286-293
[7]   A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes [J].
Cavrois, M ;
de Noronha, C ;
Greene, WC .
NATURE BIOTECHNOLOGY, 2002, 20 (11) :1151-1154
[8]  
Cenker JJ, 2017, AIDS RES HUM RETROV, V33, P1155, DOI [10.1089/aid.2017.0004, 10.1089/AID.2017.0004]
[9]   Distinct HIV-1 entry phenotypes are associated with transmission, subtype specificity, and resistance to broadly neutralizing antibodies [J].
Chikere, Kelechi ;
Webb, Nicholas E. ;
Chou, Tom ;
Borm, Katharina ;
Sterjovski, Jasminka ;
Gorry, Paul R. ;
Lee, Benhur .
RETROVIROLOGY, 2014, 11
[10]   HIV-1 envelope, integrins and co-receptor use in mucosal transmission of HIV [J].
Cicala, Claudia ;
Arthos, James ;
Fauci, Anthony S. .
JOURNAL OF TRANSLATIONAL MEDICINE, 2011, 9