Inhibition of TRPP3 by calmodulin through Ca2+/calmodulin-dependent protein kinase II

被引：0

作者：

Liu, Xiong ^{[1
]}

Wang, Yifang ^{[1
,2
]}

Weng, Ziyi ^{[1
,2
]}

Xu, Qinyi ^{[1
]}

Zhou, Cefan ^{[2
]}

Tang, JingFeng ^{[2
]}

Chen, Xing-Zhen ^{[1
,2
]}

机构：

[1] Univ Alberta, Fac Med & Dent, Dept Physiol, Membrane Prot Dis Res Grp, Edmonton, AB T6G 2H7, Canada

[2] Hubei Univ Technol, Natl Ctr Cellular Regulat & Mol Pharmaceut 111, Wuhan 430068, Hubei, Peoples R China

来源：

CELL INSIGHT | 2023年 / 2卷 / 02期

基金：

加拿大自然科学与工程研究理事会; 中国国家自然科学基金;

关键词：

TRP channel; CaM; Calcium; CaMK2; Electrophysiology; Xenopus oocyte; POLYCYSTIN-L CHANNEL; TRANSIENT RECEPTOR; EF-HAND; CA2+-DEPENDENT DESENSITIZATION; DIRECT BINDING; CALCIUM; PKD2L1; IDENTIFICATION; VOLTAGE; DOMAIN;

D O I：

10.1016/j.cellin.2023.100088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transient receptor potential (TRP) polycystin-3 (TRPP3) is a non-selective cation channel activated by Ca2+ and protons and is involved in regulating ciliary Ca2+ concentration, hedgehog signaling and sour tasting. The TRPP3 channel function and regulation are still not well understood. Here we investigated regulation of TRPP3 by calmodulin (CaM) by means of electrophysiology and Xenopus oocytes as an expression model. We found that TRPP3 channel function is enhanced by calmidazolium, a CaM antagonist, and inhibited by CaM through binding of the CaM N-lobe to a TRPP3 C-terminal domain not overlapped with the EF-hand. We further revealed that the TRPP3/CaM interaction promotes phosphorylation of TRPP3 at threonine 591 by Ca2+/CaM-dependent protein kinase II, which mediates the inhibition of TRPP3 by CaM.

引用

页数：11

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