Development of an improved reverse genetics system for avian metapneumovirus (aMPV): A novel vaccine vector protects against aMPV and infectious bursal disease virus

被引：0

作者：

Meng, Lingzhai ^{[1
]}

Chen, Yuntong ^{[1
]}

Yu, Mengmeng ^{[1
]}

Liu, Peng ^{[1
]}

Qi, Xiaole ^{[1
]}

Xue, Xiaoxiao ^{[1
]}

Guo, Ru ^{[1
]}

Zhang, Tao ^{[1
]}

Hu, Mingxue ^{[1
]}

Fan, Wenrui ^{[1
]}

Wang, Ying ^{[1
]}

Wang, Suyan ^{[1
]}

Zhang, Yanping ^{[1
]}

Liu, Yongzhen ^{[1
]}

Duan, Yulu ^{[1
]}

Cui, Hongyu ^{[1
]}

Gao, Yulong ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Avian Immunosuppress Dis Div, State Key Lab Anim Dis Control & Prevent, Harbin Vet Res Inst, Harbin 150069, Peoples R China

[2] Natl Poultry Lab Anim Resource Ctr, Harbin 150069, Peoples R China

来源：

JOURNAL OF INTEGRATIVE AGRICULTURE | 2025年 / 24卷 / 05期

关键词：

avian metapneumovirus; reverse genetics system; insertion site; protection; vector vaccines; TURKEY RHINOTRACHEITIS VIRUS; RESPIRATORY SYNCYTIAL VIRUS; ORDER DIFFERENT; FUSION PROTEIN; SUBGROUP-C; IN-VITRO; SH-GENE; CHICKENS; RECOVERY; RESCUE;

D O I：

10.1016/j.jia.2024.04.025

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Avian metapneumovirus (aMPV), a paramyxovirus, causes acute respiratory diseases in turkeys and swollen head syndrome in chickens. This study established a reverse genetics system for aMPV subtype B LN16-A strain based on T7 RNA polymerase. Full-length cDNA of the LN16-A strain was constructed by assembling 5 cDNA fragments between the T7 promoter and hepatitis delta virus ribozyme. Transfection of this plasmid, along with the supporting plasmids encoding the N, P, M2-1, and L proteins of LN16-A into BSR-T7/5 cells, resulted in the recovery of aMPV subtype B. To identify an effective insertion site, the enhanced green fluorescent protein (EGFP) gene was inserted into different sites of the LN16-A genome to generate recombinant LN16-As. The results showed that the expression levels of EGFP at the site between the G and L genes of LN16-A were significantly higher than those at the other two sites (between the leader and N genes or replacing the SH gene). To verify the availability of the site between G and L for foreign gene expression, the VP2 gene of very virulent infectious bursal disease virus (vvIBDV) was inserted into this site, and recombinant LN16-A (rLN16A-vvVP2) was successfully rescued. Single immunization of specificprotection against the virulent aMPV subtype B and vvIBDV. Establishing a reverse genetics system here provides an important foundation for understanding aMPV pathogenesis and developing novel vector vaccines.

引用

页码：1972 / 1986

页数：15

共 48 条

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