Analysis of ultrasound and cellulase for biomass accumulation and lignocellulose biodegradation of dairy manure by black soldier fly(Hermetia illucens L.)

Resource utilization of dairy manure is an issue that needs to be addressed for it to be further utilized as biomass. We explored a new comprehensive degradation strategy which combined ultrasound and cellulase for the biodegradation of dairy manure by black soldier fly. In this study, the 1.6 % cellulase group and the 14 min ultrasound group were respectively found the most effective degradation in their experiment groups. Then 1000 g dairy manure combined with the treatments of 1.6 % cellulase and 14 min ultrasound were converted by 1000 black soldier fly larvae for biomass accumulation and lignocellulose biodegradation. As a result, 5.66 g of dry larvae which contained 1.95 g of crude fat and 2.43 g of protein were obtained. The biodegradation process led to reductions in cellulose, hemicellulose, and lignin content by 15.28 %, 17.96 %, and 14.67 %. The degradation rates of cellulose, hemicellulose and lignin were 34.78 %, 32.15 % and 17.58 %. Scanning electron microscopy (SEM) and infrared spectroscopy (IR) were utilized for the mechanism analysis of this study. This study could provide a reference for the efficient degradation and utilization of dairy manure.