Colorimetric Smartphone-Based Dry Assay for the Determination of Hemoglobin

被引：0

作者：

Li, Ping ^{[1
]}

Lv, Yuan ^{[1
]}

Yang, Sainan ^{[1
]}

Xu, Keqian ^{[2
]}

Luo, Haiyong ^{[1
]}

Liu, Qian ^{[1
]}

Dai, Bin ^{[1
]}

机构：

[1] Hunan Polytech Environm & Biol, Coll Med & Technol, Hengyang, Hunan, Peoples R China

[2] Cent South Univ, Xiangya Sch Med, Changsha, Hunan, Peoples R China

来源：

ANALYTICAL LETTERS | 2025年

关键词：

Hemoglobin determination; image colorimetry; smartphone; SENSOR; IRON;

D O I：

10.1080/00032719.2025.2511884

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel smartphone-based dry assay was developed for quantitative determination of hemoglobin concentration ([Hb]) in blood. Hemoglobin ((Hb) test strips and smartphone imaging enabled analysis of multiple color parameters, with the G value providing the highest sensitivity (slope = 3.36). Analytical models based on the G value showed that the G0-G model was most robust to variations in light intensity and color temperature (maximum deviations: -48% and -7.76%, respectively). Optimal sensitivity was achieved at +1 EV exposure compensation. The regression equation was y = 4.33[Hb] - 12.3 (R-2 = 0.995, n = 3), and the detection limit was 0.62 g/dL. The assay exhibited good repeatability at low, medium, and high [Hb] levels (RSDs: 4.8%, 1.3%, 2.1%; n = 10) and strong agreement with a commercial HemoCue analyzer (R-2 = 0.910, n = 30). This method offers a cost-effective tool for on-site Hb testing.

引用

页数：13

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