Genome-Wide Identification and Expression Analysis of the AP2/ERF Transcription Factor Gene Family in Hybrid Tea Rose Under Drought Stress

被引：0

作者：

Yan, Xinyu ^{[1
]}

Huang, Wei ^{[1
]}

Liu, Cheng ^{[1
]}

Hao, Xuan ^{[1
]}

Gao, Chengye ^{[1
]}

Deng, Minghua ^{[2
]}

Wen, Jinfen ^{[1
]}

机构：

[1] Kunming Univ Sci & Technol, Fac Architecture & City Planning, Kunming 650021, Peoples R China

[2] Yunnan Agr Univ, Coll Landscape & Hort, Key Lab Vegetable Biol Yunnan Prov, Kunming 650201, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 23期

基金：

中国国家自然科学基金;

关键词：

hybrid tea rose; RhAP2/ERF; phylogenetic analysis; differential expression; drought stress; ARABIDOPSIS; SALT;

D O I：

10.3390/ijms252312849

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drought stress is an important factor that reduces plant biomass production and quality. The APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) gene family is widely involved in biological processes such as plant growth, development, and stress response. However, the characteristics of the AP2/ERF gene family in hybrid tea rose (Rosa x hybrida) and their potential functions in responding to drought stress are still unclear. In the current study, 127 AP2/ERF genes were identified in hybrid tea rose. Phylogenetic analysis showed that the corresponding 127 AP2/ERF transcription factors belonged to five subfamilies. There was a large number of cis-acting elements in the AP2/ERF gene promoters related to regulation of stress response, growth and development. By examining the RNA sequencing data in the PlantExp database, the RhAP2/ERF genes exhibiting tissue-specific and stress-responsive expression in rose were identified. Furthermore, three candidate RhAP2/ERF genes (RhDREB36, RhERF59, and RhDREB44) that might participate in drought response were determined via qRT-PCR analysis in rose cultivars under drought treatment. Subcellular localization analysis revealed that RhDREB44 was located in the nucleus. These results provide a foundation for exploring the regulatory functions of RhAP2/ERF genes in the growth and development of roses, as well as for selecting key genes for future molecular breeding.

引用

页数：19

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