TikTok is one of the most popular video-sharing social media platforms currently, with an increasing number of users posting and searching for cervical cancer-related videos. However, the quality and reliability of these videos have not been thoroughly evaluated. We conducted searches using #cervical cancer on TikTok, reviewed all included videos, and identified seven themes related to cervical cancer for analysis. We assessed the quality and reliability of the videos using the JAMA score and Modified DISCERN score. We also conducted intergroup comparative analysis of video quality and reliability for different thematic contents. A total of 100 Chinese-language videos related to cervical cancer were collected. Seven themes were established based on content, including cervical cancer patient experiences, cause of disease, symptoms, HPV-related topics, cancer treatment, laboratory and imaging examinations, and prognosis. The median JAMA score for all videos was only 1 (0, 3), and the median Modified DISCERN score was also only 1 (0, 4), indicating unsatisfactory results. When comparing video scores by different thematic contents, both scores showed statistically significant differences between groups (p < 0.001). Overall, the quality and reliability of Chinese-language cervical cancer videos on TikTok are low. Healthcare professionals should strive to improve video quality and reliability when publishing content, and patients should maintain a cautious attitude when searching for related content.