Heprotective Effect and Antioxidant of Sonchus Oleraceus Leaves Extract Against Carbon Tetrachloride (CCl4) Induced hepatotoxicity in Albino Rats

This study examined the effects of Sonchus Oleraceus extract from leaves (SO-LE) on carbon tetra chloride induced hepatotoxicity amongst albino rats. The SO-LE exhibited a high content of total phenolic and flavonoid compounds accounting for 127.37 +/- 0.56 mg GAE/g (Gallic Acid Equivalent./gram )extract and 74.23 +/- 0.55 mg QE/g( quercetin equivalent ) extract, respectively. SO-LE exhibited strong DPPH( 1,1diphenyl,2 picrylhydrazyl) scavenging activity with an inhibition ratio of 43.07%. For the in vivo assay, 24 adult albino rats were separated into four groups (each of six rats). First group served as the negative group, while the second group received carbon tetrachloride (CCl4) as the positive control. Third group received CCl4 with 100 mg kg-1 SO-LE, while the fourth group received CCl4 with 200 mg kg-1 SO-LE for eight weeks (CCl4 concentration was 1 mL kg-1). Rat lipid profiles, peroxidation, and indicators of liver and kidney function were evaluated. Treatment with 200 mg kg-1 SO-LE reduced creatinine, urea, and uric acid levels to 0.87, 38, and 3.8 mg dL-1, respectively. After eight weeks of treatment, levels of TC, TAG, and LDL were decreased to 129.7, 146.3, and 62.7 mg L-1, respectively. SOLE reduced AST and ALT activity and decreased malondialdehyde levels, whereas CAT, GSH, and SOD levels increased in SO-LE-treated albino rats. The liver's histopathological analysis revealed that the SO-LE treatment decreased necrosis, cytoplasmic vacuolization, and fatty acid degradation. In conclusion, SO-LE protected albino rats against CCl4-induced hepatotoxicity, a protective effect that may have been enhanced by the antioxidant properties of SO-LE extract. The objective of this study was to investigate the preventive effects of SO-LE on rats' fatty livers caused by carbon tetra chloride.