Induced neural stem cells ameliorate blood-brain barrier injury by modulating the calcium signaling pathway of astrocyte in cerebral ischemia-reperfusion rats

被引：0

作者：

Liang, Xueyun ^{[1
]}

Cao, Chuanshang ^{[1
]}

Liu, Ningmei ^{[1
]}

Chen, Dongmei ^{[1
]}

Liu, Ting ^{[1
]}

Ma, Haibin ^{[1
]}

Liu, Jiaxin ^{[1
]}

Wu, Taojuan ^{[1
]}

Niu, Jianguo ^{[2
]}

机构：

[1] Ningxia Med Univ, Inst Med Sci, Key Lab Ningxia Stem Cell & Regenerat Med, Gen Hosp, Yinchuan, Peoples R China

[2] Ningxia Med Univ, Sch Basic Med Sci, Ningxia Key Lab Cerebrocranial Dis, Yinchuan, Peoples R China

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2025年 / 13卷

基金：

中国国家自然科学基金;

关键词：

induced neural stem cells; BBB; astrocytes; calcium signaling pathways; cerebral ischemia-reperfusion; AQUAPORIN-4; EXPRESSION; LOCALIZATION; PROTECTS;

D O I：

10.3389/fcell.2025.1611226

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background Neural stem cells offer new hope for ischemic stroke patients on the basis of their potential to reverse neurological sequelae, but it is still difficult to obtain sufficient neural stem cells in the clinic. We induced human placental mesenchymal stem cells to neural stem cells (iNSCs), the therapeutic effects and possible mechanisms of iNSCs in ischemic stroke were observed in this study.Results Transplanted iNSCs improved neurological deficits, increased the integrity of blood-brain barrier (BBB) structure and its related proteins expression level in middle cerebral artery occlusion/reperfusion (MCAO/R) rats. The in vitro study demonstrated that iNSCs treatment inhibited Ca2+ influx in oxygen-glucose deprived (OGD)-damaged astrocytes. Additionally, iNSCs downregulated the expression level of pCaMK-II, increased the expression level of superoxide dismutase, and inhibited the expression of caspase 9 in both brain of MCAO/R rats and OGD-damaged astrocytes.Conclusion iNSCs transplantation improved BBB function by modulating calcium signaling pathway of astrocyte in MCAO/R rats, which proved iNSCs may be a new promising neural stem cells origin for the treatment of cerebral ischemia-reperfusion injury.

引用

页数：15

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