Exogenous administration of heparin-binding epidermal growth factor-like growth factor improves erectile function in mice with bilateral cavernous nerve injury

被引:0
作者
Vo, Minh Nhat [1 ,2 ]
Kwon, Mi-Hye [1 ,2 ]
Liu, Fang-Yuan [1 ,2 ]
Fridayana, Fitri Rahma [1 ,2 ,3 ]
Huang, Yan [1 ,2 ,3 ]
Hong, Soon-Sun [3 ]
Kang, Ju-Hee [4 ]
Yin, Guo Nan [1 ,2 ]
Ryu, Ji-Kan [1 ,2 ,3 ]
机构
[1] Inha Univ, Natl Res Ctr Sexual Med, Sch Med, Incheon 22332, South Korea
[2] Inha Univ, Sch Med, Dept Urol, Incheon 22332, South Korea
[3] Inha Univ, Coll Med, Dept Biomed Sci, Program Biomed Sci & Engn, Incheon 22332, South Korea
[4] Inha Univ, Coll Med, Med Toxicol Res Ctr, Dept Pharmacol, Incheon 22212, South Korea
来源
ASIAN JOURNAL OF ANDROLOGY | 2025年
基金
新加坡国家研究基金会;
关键词
cavernous nerve injury; erectile dysfunction; HB-EGF; nerve regeneration; reactive oxygen species; FACTOR HB-EGF; SHOCK-WAVE THERAPY; DIFFERENTIATION; PROLIFERATION; DYSFUNCTION; EXPRESSION; SYSTEM; ROLES;
D O I
10.4103/aja2024125
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Prostate cancer is the second most common malignancy and the sixth leading cause of cancer-related death in men worldwide. Radical prostatectomy (RP) is the standard treatment for localized prostate cancer, but the procedure often results in postoperative erectile dysfunction (ED). The poor efficacy of phosphodiesterase 5 inhibitors after surgery highlights the need to develop new therapies to enhance cavernous nerve regeneration and improve the erectile function of these patients. In the present study, we aimed to examine the potential of heparin-binding epidermal growth factor-like growth factor (HB-EGF) in preserving erectile function in cavernous nerve injury (CNI) mice. We found that HB-EGF expression was reduced significantly on the 1st day after CNI in penile tissue. Ex vivo and in vitro studies showed that HB-EGF promotes major pelvic ganglion neurite sprouting and neuro-2a (N2a) cell migration. In vivo studies showed that exogenous HB-EGF treatment significantly restored the erectile function of CNI mice to 86.9% of sham levels. Immunofluorescence staining showed that mural and neuronal cells were preserved by inducing cell proliferation and reducing apoptosis and reactive oxygen species production. Western blot analysis showed that HB-EGF upregulated protein kinase B and extracellular signal-regulated kinase activation and neurotrophic factor expression. Overall, HB-EGF is a major promising therapeutic agent for treating ED in postoperative RP.
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页数:10
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