Rapid Determination of 107 Illegally Added Nafils in Foods by Ultra-high Performance Liquid Chromatography-High Resolution Mass Spectrometry

被引：0

作者：

Feng, Feng ^{[1
,2
]}

Chang, Qizhi ^{[1
,2
,3
]}

Sun, Xiao ^{[1
,2
,4
]}

Du, Rongzhu ^{[1
,2
,3
]}

Zhang, Feng ^{[1
,2
]}

机构：

[1] Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing

[2] Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing

[3] School of Pharmacy, China Medical University, Shenyang

[4] Xi’an Institute for Food and Drug Control, Xi’an

来源：

Shipin Kexue/Food Science | 2025年 / 46卷 / 10期

关键词：

high-resolution mass spectrometry; illegal additives; nafils; ultra-high performance liquid chromatography;

D O I：

10.7506/spkx1002-6630-20240808-067

中图分类号：

学科分类号：

摘要：

A rapid method for the determination 107 illegally added nafils in compressed candies, honey and beverage was developed by ultra-high performance liquid chromatography coupled with quadrupole orbitrap high-resolution mass spectrometry. The samples were extracted with methanol and separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with gradient elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water. The detection was conducted in both positive and negative ion modes, and a full MS scan/data dependent MS2 mode was used to collect data. The 107 nafils showed good linearity in the concentration range of 4–300 μg/L, with correlation coefficients (R2) greater than 0.99. The limits of detection (LODs) were 0.1–1 mg/kg, and the limits of quantification (LOQs) were 0.2–2 mg/kg. The average recoveries ranged from 82.5% to 117.0% at three spiked levels of 2, 5, 10 mg/kg, with relative standard deviations of 0.9%–10.0% (n = 6). This method was fast, accurate, sensitive, and suitable for the rapid identification and quantification of illegal additives in foods. © 2025 Chinese Chamber of Commerce. All rights reserved.

引用

页码：290 / 298

页数：8

共 14 条

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