Athyrium multidentatum (Doll.) Ching polysaccharide coated zein nanoparticles for oral delivery of resveratrol: Fabrication, characterization, stability and in vitro digestion

Resveratrol (Res) rapidly degrades and has hydrophobic properties with low bioavailability. Encapsulating in nanoparticles can stabilize its physicochemical properties. In this study, polysaccharides from Athyrium multidentatum (Doll.) Ching (AMC) were extracted and purified by AEC to obtain fractions AMCP-1 and AMCP-2, which were mainly composed of mannose, galactose and glucose. Res was encapsulated in nanoparticles prepared by zein (RZ) or AMCP-2 combined with zein (RZA). Particle size of RZA produced when Res addition was 3 mg and zein to AMCP-2 ratio was 1:3 was 189.66 nm, with a Polymer Dispersity Index (PDI) of 0.058 and a zeta-potential of-21.23 mV. Infrared, Raman, and fluorescence spectroscopy studies revealed that hydrophobic forces, hydrogen bonding, and electrostatic interactions were main inermolecular force among zein, Res, and AMCP-2. Stability studies demonstrated that RZA exhibited improved thermal and ionic strength stability and storage stability. SEM results showed RZ had a spherical morphology and smooth surface, while RZA had a rough surface and uniform distribution. Scavenging activity against ABTS+ of Res in RZA was significantly enhanced (p < 0.05). The bioaccessibility of Res in RZA increased from 39.77 % to 51.35 % after gastrointestinal digestion. In summary, nanoparticles prepared by AMCP and zein have potential applications in hydrophobic substances loading.