New Steroids Obtained from Ailanthus altissima Leaves Inhibit the Invasive Bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas syringae pv. maculicola

被引：0

作者：

Yang, Yuhong ^{[1
]}

Wu, Yue ^{[1
]}

Gao, Zhengyi ^{[1
]}

Liu, Zhixiang ^{[1
]}

Hua, Juan ^{[1
]}

Luo, Shihong ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Biosci & Biotechnol, Engn Res Ctr Protect & Utilizat Plant Resources, Shenyang 110866, Peoples R China

来源：

MOLECULES | 2025年 / 30卷 / 12期

关键词：

invasive bacteria; Ailanthus altissima; steroids; Xanthomonas oryzae pv. oryzae; Pseudomonas syringae pv. maculicola ES4326; antibacterial activity; CONSTITUENTS; COMPONENTS; SHOOTS; BARK;

D O I：

10.3390/molecules30122576

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Invasive bacteria have caused tremendous losses to global ecosystems and agricultural production, yet effective control measures remain elusive. Plant specialized metabolites are being investigated as an important source of antimicrobial active substances. And Ailanthus altissima is an abundant tree widespread throughout Northeast China. In this study, we identified 21 compounds from A. altissima leaves, including steroids, terpenes, phenolics, and coumarins. Two new steroidal compounds, ailanstigol A (1) and ailanstigol B (2), and one new coumarin (2 ' R,3 ' R)-7-(2 ',3 ',6 '-trihydroxy-3 '-methylhexyloxy)-6,8-dimethoxycoumarin (3) were isolated. Antibacterial screening revealed that compounds 1 and 2 exhibited inhibitory activity against two invasive bacteria, Xanthomonas oryzae pv. oryzae PXO 71A and PXO 86A and Pseudomonas syringae pv. maculicola ES4326. Further mechanistic screening unveiled that the steroidal compounds 1 and 2 may inhibit bacterial growth and reproduction by reducing cell viability, disrupting the cell membrane and increasing protein leakage, and inhibiting biofilm formation. In summary, our results enriched the known chemical diversity of A. altissima and provided a foundation for investigating the mechanisms by which steroidal compounds inhibit invasive bacterial growth.

引用

页数：16

共 49 条

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