A Convenient Fluorogenic Detection Strategy for Phosphorothioate Modification of DNA Through Photocatalytic Oligonucleotide-Templated Reaction

被引：0

作者：

Jing, Nannan ^{[1
]}

Qin, Yantian ^{[2
]}

Fan, Xinli ^{[2
]}

Wang, Qian ^{[2
]}

Wang, Jing ^{[2
]}

You, Fuping ^{[1
]}

Tang, Xinjing ^{[2
]}

机构：

[1] Peking Univ, Sch Basic Med Sci, Dept Pharmacol, 38 Xueyuan Rd, Beijing 100191, Peoples R China

[2] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, 38 Xueyuan Rd, Beijing 100191, Peoples R China

来源：

BIOMOLECULES | 2025年 / 15卷 / 06期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

fluorescence probe; phosphorothioate modifications; sequence specificity; oligonucleotide-templated reactions; fluorogenic oligonucleotide probes;

D O I：

10.3390/biom15060752

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA phosphorothioate (PT) modifications, characterized by the replacement of a non-bridging phosphate oxygen atom with a sulfur atom, are widely observed in bacterial genomes. Sensitive detection of phosphorothioate is crucial for elucidating their biological roles and functions. Herein, we developed an innovative method that leverages oligonucleotide-templated reactions (OTRs) and fluorogenic oligonucleotide probes. By optimizing temperature, probe sequence length, and the relative distance between PT position and the fluorophore probe, we achieved sensitive detection for DNA PT modifications through fluorogenic signal amplification, which provides an efficient and cost-effective approach for sensitive detection of phosphorothioate-modified DNA.

引用

页数：11

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