Direct electron transfer chemistry of redox-active enzymes: applications in biosensor development

被引：1

作者：

Ahmad, Fawad ^{[1
]}

Zahid, Shafaq ^{[1
]}

Khan, Muhammad Imran ^{[2
]}

Shanableh, Abdallah ^{[2
,3
]}

Farooq, Nosheen ^{[4
]}

Rao, Komal Ali ^{[5
]}

Taj, Muhammad Babar ^{[6
]}

Manzoor, Suryyia ^{[7
]}

Voskressensky, Leonid G. ^{[8
]}

Luque, Rafael ^{[8
,9
]}

机构：

[1] Univ Wah, Dept Chem, Wah Cantt, Pakistan

[2] Univ Sharjah, Res Inst Sci & Engn RISE, Sharjah, U Arab Emirates

[3] Australian Univ, Sci Res Ctr, Mubarak Al Abdullah, Kuwait

[4] Govt Sadiq Coll Women Univ, Dept Chem, Bahawalpur, Pakistan

[5] Bahauddin Zakariya Univ, Inst Phys, Multan, Pakistan

[6] Islamia Univ Bahawalpur, Inst Chem, Bahawalpur, Pakistan

[7] Bahauddin Zakariya Univ, Inst Chem Sci, Multan, Pakistan

[8] Peoples Friendship Univ Russia RUDN Univ, Moscow, Russia

[9] Univ ECOTEC, Ctr Estudios Desarrollo Sostenible CEDS, Guayaquil, Ecuador

来源：

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR | 2025年 / 19卷 / 03期

关键词：

prosthetic group; direct electron transfer; redox active; biosensor; co-factor; GLUCOSE BIOFUEL CELL; WALLED CARBON NANOTUBE; CELLOBIOSE DEHYDROGENASE; DIRECT ELECTROCHEMISTRY; CYTOCHROME-C; FRUCTOSE DEHYDROGENASE; ELECTROCATALYTIC ACTIVITY; HETEROLOGOUS EXPRESSION; COULOMBIC EFFICIENCY; GRAPHENE OXIDE;

D O I：

10.1002/bbb.2742

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The direct electron transfer (DET) process, which does not require a mediator, is highly advantageous and holds significant potential for application in biosensors. Enzyme-based biosensors are simple, cost-efficient, and easy to use. This review highlights key enzymes with redox-active sites that are suitable for DET processes using various modified electrodes and their applications in biosensors. The review discusses enzyme classification, distinguishing between single-cofactor and multi-cofactor enzymes according to their number of prosthetic groups, with a focus on internal electron transfer processes and the role of redox-active centers. Methods for enzyme immobilization on electrodes are explored with an emphasis on their impact on electron transfer rates in biosensors. A comparison of sensitivity, electron transfer rates, and the linear detection range of molecules (e.g., glucose and lactose) is provided to illustrate the impact of different immobilization techniques on biosensor performance.

引用

页码：963 / 981

页数：19

共 139 条

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