Recent advances in bioreceptor-based sensing for extracellular vesicle analysis

Extracellular vesicles (EVs) are nanoscale, membrane-bound structures secreted by various cell types into biofluids. They show great potential as biomarkers for disease diagnostics, owing to their ability to carry molecular cargo that reflects their cellular origin. However, the inherent heterogeneity of EVs in terms of size, composition, and source presents significant challenges for reliable detection and analysis. Recent advances in bioreceptorbased biosensor technologies provide promising solutions by offering high sensitivity and specificity in EV detection and characterization. These technologies address the limitations of conventional methods, such as ultracentrifugation and bulk analysis. Biosensors utilizing antibodies, aptamers, peptides, lectins, and molecularly imprinted polymers enable precise detection of EV subpopulations by targeting specific EV surface markers, including proteins, lipids, and glycans. Additionally, these biosensors support multiplexed and real-time analysis while preserving the structural integrity of EVs. This review highlights the transformative potential of combining modern biosensing tools with bioreceptor technologies to advance EV research and diagnostics, paving the way for innovations in disease diagnostics and therapeutic monitoring.