Exploring the mechanisms and mode of action of bioactive compounds from marine Streptomyces albidoflavus against breast carcinoma cells

被引：0

作者：

Abir M. Shata ^{[1
]}

Mokhtar Saeed Rejili ^{[2
]}

Manal M. El-Naggar ^{[1
]}

Samy A. El-Asser ^{[3
]}

Ahmed A. Saleh ^{[4
]}

Elsayed E. Hafez ^{[5
]}

Faouzi Haouala ^{[6
]}

Amany S. Youssef ^{[2
]}

机构：

[1] National Institute of Oceanography and Fisheries,Department of Biology, College of Sciences

[2] NIOF,Faculty of Science, Department of Botany and Microbiology

[3] Imam Mohammad Ibn Saud Islamic University (IMSIU),College of Animal Science & Technology

[4] Alexandria University,Faculty of Agriculture (Al

[5] Yangzhou University,Shatby), Animal and Fish Production Department

[6] Alexandria University,Department of Plant Protection and Biomolecular Diagnosis

[7] ALCRI City of Scientific Research and Technological Applications,undefined

来源：

BMC Biotechnology | / 25卷 / 1期

关键词：

Marine ; Anti-breast carcinoma; MTTbioassay; SRBbioassay; Apoptosis;

D O I：

10.1186/s12896-025-00991-5

中图分类号：

学科分类号：

摘要：

Breast cancer remains one of the most lethal diseases for women worldwide. Marine populations are considered a vast reservoir for novel bioactive metabolites, particularly marine Actinomycetes, which are known to produce various bioactive compounds with antitumour, antibacterial, and antifungal properties. A promising new marine strain was isolated and identified as Streptomyces albidoflavus strain EgyAB2 (16 S rRNA gene sequence accession number ON680945.1). The anticancer activity of the extracted compounds was tested in the MCF7 cell line using a sulforhodamine B (SRB) bioassay, which revealed an IC50 of 0.36 µg/ml compared to the chemotherapeutic drug 5-fluorouracil (0.35 µg/ml). Additionally, the anticancer activity was confirmed by a dimethyl-thiazol-diphenyl-tetrazolium bromide (MTT) bioassay, which showed an IC50 of 17.46 µg/ml. The mode of action of the treated breast carcinoma (apoptotic effect) was studied via qRT-PCR, revealing a significant role in anticancer treatment. Although the extracted compounds exhibited high antioxidant activity in the diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, they presented an IC50 of 8.92 µg/ml and an inhibition percentage of 56.08%. Chemical characterisation was performed via GC‒MS, 1 H-NMR, and FTIR spectroscopy analyses, revealing the presence of 2-D N-methyl imidazole, 2-nonadecene, 1-D-2-methyl imidazole, and propane dinitrile, all of which exhibit antitumour activity.

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